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Title: Genotyping species of the Sporothrix schenckii complex by PCR-RFLP of calmodulin
Authors: Rodrigues, Anderson Messias [UNIFESP]
Hoog, G. Sybren de
Camargo, Zoilo Pires de [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
CBS KNAW Fungal Biodivers Ctr
Keywords: Sporothrix brasiliensis
Sporothrix schenckii
Molecular identification
Issue Date: 1-Apr-2014
Publisher: Elsevier B.V.
Citation: Diagnostic Microbiology and Infectious Disease. New York: Elsevier B.V., v. 78, n. 4, p. 383-387, 2014.
Abstract: Sporotrichosis is one of the most common subcutaneous mycosis in Latin America and is caused by 4 pathogenic thermodimorphic fungi in the genus Sporothrix. From both therapeutic and epidemiological perspectives, it is essential to identify the causative agents down to the species level. Traditional parameters may overlap among closely related species, and we propose polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as an alternative approach. in the present study, the calmodulin gene was amplified and digested with Hhal to yield 5 different electrophoretic patterns representing all medically important Sporothrix species: Sporothrix brasiliensis, Sporothrix schenckii sensu stricto, Sporothrix globosa, and Sporothrix luriei. the PCR-RFLP protocol described here is a simple and inexpensive method and is highly suitable for accurate routine genotyping of relevant Sporothrix species. (C) 2014 Elsevier Inc. All rights reserved.
ISSN: 0732-8893
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