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dc.contributor.authorVidal, C. M. P.
dc.contributor.authorTjaderhane, L.
dc.contributor.authorScaffa, P. M.
dc.contributor.authorTersariol, I. L. [UNIFESP]
dc.contributor.authorPashley, D.
dc.contributor.authorNader, H. B. [UNIFESP]
dc.contributor.authorNascimento, F. D.
dc.contributor.authorCarrilho, M. R.
dc.identifier.citationJournal of Dental Research. Thousand Oaks: Sage Publications Inc, v. 93, n. 3, p. 269-274, 2014.
dc.description.abstractDegradation of dentin matrix components within caries dentin has been correlated with the activity of host-derived proteases, such as matrix metalloproteases (MMPs) and cysteine cathepsins (CTs). Since this relationship has not been fully established, we hypothesized that the abundance of MMPs and CTs in caries-affected dentin must be higher than in intact dentin. To test this premise, we obtained 5 slices (200 mu m) from 5 intact teeth and from 5 caries-affected teeth (1 slice/tooth) and individually incubated them with primary antibodies for CT-B, CT-K, MMP-2, or MMP-9. Negative controls were incubated with pre-immune serum. Specimens were washed and re-incubated with the respective fluorescent secondary antibody. Collagen identification, attained by the autofluorescence capture technique, and protease localization were evaluated by multi-photon confocal microscopy. the images were analyzed with ZEN software, which also quantitatively measured the percentages of collagen and protease distribution in dentin compartments. the abundance of the test enzymes was markedly higher in caries-affected than in intact dentin. CT-B exhibited the highest percentage of co-localization with collagen, followed by MMP-9, MMP-2, and CT-K. the high expression of CTs and MMPs in caries-affected teeth indicates that those host-derived enzymes are intensely involved with caries progression.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.publisherSage Publications Inc
dc.relation.ispartofJournal of Dental Research
dc.rightsAcesso restrito
dc.subjectfluorescent immunohistochemistryen
dc.subjectdental cariesen
dc.subjectdentin cysteine proteasesen
dc.titleAbundance of MMPs and Cysteine Cathepsins in Caries-affected Dentinen
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniv Oulu
dc.contributor.institutionOulu Univ Hosp
dc.contributor.institutionUniv Mogi das Cruzes
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionGeorgia Regents Univ
dc.contributor.institutionUniv Bandeirante São Paulo
dc.contributor.institutionUniv Western Ontario
dc.description.affiliationUniv Estadual Campinas, Piracicaba Dent Sch, Dent Mat Area, Dept Restorat Dent, Piracicaba, Brazil
dc.description.affiliationUniv Oulu, Inst Dent, Oulu, Finland
dc.description.affiliationOulu Univ Hosp, Oulu, Finland
dc.description.affiliationUniv Mogi das Cruzes, Ctr Interdisciplinar Invest Bioquim, Mogi Das Cruzes, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biochem, São Paulo, Brazil
dc.description.affiliationGeorgia Regents Univ, Dept Oral Biol, Coll Dent Med, Augusta, GA USA
dc.description.affiliationUniv Bandeirante São Paulo, Biomat Res Grp, São Paulo, Brazil
dc.description.affiliationUniv Bandeirante São Paulo, Div Biotechnol, São Paulo, Brazil
dc.description.affiliationUniv Western Ontario, Schulich Sch Med & Dent, London, ON, Canada
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biochem, São Paulo, Brazil
dc.description.sponsorshipIDFAPESP: 2007/54618-4
dc.description.sponsorshipIDFAPESP: 2011/12226-8
dc.description.sponsorshipIDFAPESP: 2013/05822-9
dc.description.sponsorshipIDFAPESP: 2009/13652-0
dc.description.sponsorshipIDCNPq: 306100/2010-0
dc.description.sourceWeb of Science
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