Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/37290
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dc.contributor.authorRusso, Cristiano Teodoro
dc.contributor.authorAlkmim, Wagner [UNIFESP]
dc.contributor.authorMunerato, Patricia [UNIFESP]
dc.contributor.authorZukurov, Jean [UNIFESP]
dc.contributor.authorMaricato, Juliana Terzi [UNIFESP]
dc.contributor.authorSucupira, M. Cecilia [UNIFESP]
dc.contributor.authorDiaz, Ricardo S. [UNIFESP]
dc.contributor.authorJanini, Luiz Mario [UNIFESP]
dc.date.accessioned2016-01-24T14:35:07Z
dc.date.available2016-01-24T14:35:07Z
dc.date.issued2014-01-01
dc.identifierhttp://dx.doi.org/10.1159/000362415
dc.identifier.citationIntervirology. Basel: Karger, v. 57, n. 5, p. 277-288, 2014.
dc.identifier.issn0300-5526
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/37290
dc.description.abstractHuman immunodeficiency virus type 1 (HIV-1) genetic diversity is one of the most important features of HIV-1 infections and the result of error accumulation during reverse transcription and of high viral turnover. HIV-1 reverse transcription is influenced by factors such as the level of nucleotides and/or the cellular activation state. HIV-1 diversity was investigated after 48 h of viral propagation in peripheral blood mononuclear cells (PBMCs) obtained from healthy donors in three different cell culture conditions: (1) resting PBMCs, (2) simultaneous infection and PBMC activation, and (3) PBMC activation 72 h before infection. Cellular DNA was extracted and proviruses of each culture condition were amplified. Single-genonne PCR clones were obtained and the protease and reverse transcriptase of the pol gene were sequenced. An elevated number of nucleotide substitutions in all three culture conditions were observed. in condition 1, the mutational rate observed ranged from 1.0 x 10(-3) to 2.1 x 10(-2), the genetic diversity was 0.6%, and hypermutation was observed in 7.1% of sequenced clones. in condition 2, the mutational rate ranged from 1.0 x 10(-3) to 1.0 x 10(-2), the genetic diversity was 0.8%, and hypermutation affected 6.7% of clones. in condition 3, the mutational rate ranged from 2.8 x 10(-3) to 1.1 x 10(-2), the genetic diversity was 1%, and 5.9% of clones were hypermutated. Substitutions occurred more frequently in some specific nucleotide stretches, and a common pattern for substitutions in all the different conditions was identified. There was a significant accumulation of mutations during the initial periods of in vitro HIV-1 propagation irrespective of culture conditions. the rapid accumulation of virus diversity might represent a viral strategy when colonizing new hosts. Complementary studies are necessary to allow for a better understanding of the initial periods of infection, which represent a crucial event related to disease progression. (C) 2014 S. Karger AG, Baselen
dc.format.extent277-288
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofIntervirology
dc.rightsAcesso restrito
dc.subjectHIV-1en
dc.subjectPol regionen
dc.subjectViral propagationen
dc.subjectPeripheral blooden
dc.subjectMononuclear cellsen
dc.subjectMutationen
dc.subjectGenetic diversityen
dc.titleHigh Rates of Human Immunodeficiency Virus Type 1 Mutational Profiles by Single-Genome Amplification after 48-Hour Propagation in Peripheral Blood Mononuclear Cells at Different Levels of Cell Activationen
dc.typeArtigo
dc.rights.licensehttp://www.karger.com/Services/RightsPermissions
dc.contributor.institutionPontif Catholic Univ Parana
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationPontif Catholic Univ Parana, Med Course, Discipline Immunol, Londrina, Parana, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Infect Dis Div Med, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, UNIFESP, Dept Micro Immuno & Parasitol, Discipline Microbiol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Infect Dis Div Med, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, UNIFESP, Dept Micro Immuno & Parasitol, Discipline Microbiol, São Paulo, Brazil
dc.identifier.doi10.1159/000362415
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000340812800005
Appears in Collections:Artigo

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