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Title: Umbilical cord mesenchymal stem cells labeled with multimodal iron oxide nanoparticles with fluorescent and magnetic properties: application for in vivo cell tracking
Authors: Sibov, Tatiana Tais [UNIFESP]
Pavon, Lorena Favaro [UNIFESP]
Miyaki, Liza Aya
Mamani, Javier Bustamante
Nucci, Leopoldo P. [UNIFESP]
Alvarim, Larissa T.
Silveira, Paulo H.
Marti, Luciana C.
Gamarra, L. F. [UNIFESP]
Hosp Israelita Albert Einstein
Universidade Federal de São Paulo (UNIFESP)
Fac Ciencias Med Santa Casa São Paulo
Keywords: mesenchymal stem cells
multimodal iron oxide nanoparticles
magnetic resonance imaging
Parkinson's disease
Issue Date: 1-Jan-2014
Publisher: Dove Medical Press Ltd
Citation: International Journal of Nanomedicine. Albany: Dove Medical Press Ltd, v. 9, p. 337-350, 2014.
Abstract: Here we describe multimodal iron oxide nanoparticles conjugated to Rhodamine-B (MION-Rh), their stability in culture medium, and subsequent validation of an in vitro protocol to label mesenchymal stem cells from umbilical cord blood (UC-MSC) with MION-Rh. These cells showed robust labeling in vitro without impairment of their functional properties, the viability of which were evaluated by proliferation kinetic and ultrastructural analyzes. Thus, labeled cells were infused into striatum of adult male rats of animal model that mimic late onset of Parkinson's disease and, after 15 days, it was observed that cells migrated along the medial forebrain bundle to the substantia nigra as hypointense spots in T2 magnetic resonance imaging. These data were supported by short-term magnetic resonance imaging. Studies were performed in vivo, which showed that about 5 x 105 cells could be efficiently detected in the short term following infusion. Our results indicate that these labeled cells can be efficiently tracked in a neurodegenerative disease model.
ISSN: 1178-2013
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