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Title: IV Brazilian Guidelines for autoantibodies on HEp-2 cells
Authors: Francescantonio, Paulo Luiz Carvalho
Cruvinel, Wilson de Melo [UNIFESP]
Dellavance, Alessandra [UNIFESP]
Andrade, Luiz Eduardo Coelho [UNIFESP]
Taliberti, Ben Hur
Von Mühlen, Carlos Alberto
Bichara, Carlos David Araújo
Bueno, Cleonice
Mangueira, Cristóvão Luis Pitangueiras
Carvalho, Darlene Gonçalves
Bonfá, Eloisa Silva Dutra de Oliveira
Brito, Fabiano de Almeida
Araújo, Flávia Ikeda e
Rego, Jozelia
Pereira, Kaline Medeiros Costa
Anjos, Lisiane Maria Enriconi dos
Bissoli, Maria de Fatima
Santiago, Mittermayer Barreto
Maluf, Natalya Zaidan
Alvarenga, Rossana Rassi
Neves, Suzane Pretti Figueiredo
Valim, Valeria
Santos, Wilton Silva dos [UNIFESP]
PUC Goias
Fleury Med & Saude
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Uberlândia (UFU)
Clin Doencas Reumat Porto Alegre
Amaral Costa Med Diagnost
Universidade de São Paulo (USP)
Hosp Israelita Albert Einstein
Inst Hermes Pardini
Universidade Federal de Minas Gerais (UFMG)
Univ Catolica Brasilia
Universidade Federal de Goiás (UFG)
Univ Sul Santa Catarina UNISUL
Univ Vale do Itajai UNIVALE
Univ Fed Espirito Santo
Escola Super Ciencias Saude Dist Fed
Lab Sabin
Keywords: Autoantibodies
HEp-2 cells
Antinuclear antibodies
Indirect immunofluorescence
ANA consensus
Issue Date: 1-Jan-2014
Publisher: Elsevier B.V.
Citation: Revista Brasileira de Reumatologia. New York: Elsevier B.V., v. 54, n. 1, p. 44-50, 2014.
Abstract: Objective: the Fourth Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells (ANA) was held in Vitoria, Espirito Santo, and aimed to discuss strategies and recommendations about the technique, standardization, interpretation and quality control of the indirect immunofluorescence reaction on HEp-2 cells.Methods: Twenty three ANA experts from university centers and private laboratories in different areas from Brazil discussed and agreed upon recommendations for the fourth edition of the Brazilian Consensus for Autoantibodies Screening in HEp-2 Cells.Results and conclusion: the 4th ANA Consensus included three novel patterns into the existing algorithm (cytoplasmic Rods and Rings, nuclear Quasi-homogeneous, and CENP-F). Emphasis was given to the need of attention in describing the peculiar mixed pattern elicited by anti-DNA topoisomerase I (Scl-70) autoantibodies, comprising nuclear fine specked, nucleolar homogeneous pattern, NOR staining in metaphase plates, and cytoplasmic fine speckled patterns. the group also emphasized the need for continuous quality control in indirect immunofluorescence assays, the establishment of screening dilutions, as well as conjugate titration. An alert was made regarding the heterogeneity of commercial kits in defining patterns and the use of solid phase methodologies to determine the presence of autoantibodies. (C) 2014 Elsevier Editora Ltda. All rights reserved.
ISSN: 0482-5004
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