Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/36802
Title: Sialoglycoproteins in Morphological Distinct Stages of Mucor polymorphosporus and their Influence on Phagocytosis by Human Blood Phagocytes
Authors: Almeida, Catia Amancio
Campos-Takaki, Galba Maria de
Portela, Maristela Barbosa
Travassos, Luiz R. [UNIFESP]
Alviano, Celuta Sales
Alviano, Daniela Sales
Universidade Federal do Rio de Janeiro (UFRJ)
Univ Catolica Pernambuco
Universidade Federal Fluminense (UFF)
Universidade Federal de São Paulo (UNIFESP)
Keywords: Sialoglycoproteins
Mucor polymorphosporus
Mucor spp.
Mycelium
Spore
Yeast
Issue Date: 1-Oct-2013
Publisher: Springer
Citation: Mycopathologia. Dordrecht: Springer, v. 176, n. 3-4, p. 183-189, 2013.
Abstract: The possible role of sialic acids in host cells-fungi interaction and their association with glycoproteins were evaluated using a clinical isolate of the dimorphic fungus Mucor polymorphosporus. Lectin-binding assays with spores and yeast cells denoted the presence of surface sialoglycoconjugates containing 2,3- and 2,6-linked sialylglycosyl groups. Western blotting with peroxidase-labeled Limulus polyphemus agglutinin revealed the occurrence of different sialoglycoprotein types in both cell lysates and cell wall protein extracts of mycelia, spores, and yeasts of M. polymorphosporus. Sialic acids contributed to the surface negative charge of spores and yeast forms as evaluated by adherence to a cationic substrate. Sialidase-treated spores were less resistant to phagocytosis by human neutrophils and monocytes from healthy individuals than control (untreated) fungal suspensions. the results suggest that sialic acids are terminal units of various glycoproteins of M. polymorphosporus, contributing to negative charge of yeasts and spore cells and protecting infectious propagules from destruction by host cells.
URI: http://repositorio.unifesp.br/handle/11600/36802
ISSN: 0301-486X
Other Identifiers: http://dx.doi.org/10.1007/s11046-013-9692-6
Appears in Collections:Em verificação - Geral

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