Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/36790
Title: Behavior of human immunoglobulin G adsorption onto immobilized Cu(II) affinity hollow-fiber membranes
Authors: Borsoi-Ribeiro, Mariana
Lazzarotto Bresolin, Igor Tadeu [UNIFESP]
Vijayalakshmi, Mookambeswaran
Alves Bueno, Sonia Maria
Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Univ Technol Compiegne
VIT Univ Vellore
Keywords: human IgG
IMAC
Cu(II)
tris(2-aminoethyl)amine
affinity membranes
adsorption
Issue Date: 1-Oct-2013
Publisher: Wiley-Blackwell
Citation: Journal of Molecular Recognition. Hoboken: Wiley-Blackwell, v. 26, n. 10, p. 514-520, 2013.
Abstract: Iminodiacetic acid (IDA) and tris(2-aminoethyl)amine (TREN) chelating ligands were immobilized on poly(ethylene vinyl alcohol) (PEVA) hollow-fiber membranes after activation with epichlorohydrin or butanediol diglycidyl ether (bisoxirane). the affinity membranes complexed with Cu(II) were evaluated for adsorption of human immunoglobulin G (IgG). the effects of matrix activation and buffer system on adsorption of IgG were studied. Isotherms of batch IgG adsorption onto finely cut membranes showed that neither of the chelates, IDA-Cu(II) or TREN-Cu(II), had a Langmuirean behavior with negative cooperativity for IgG binding. A comparison of equilibrium and dynamic maximum capacities showed that the dynamic capacity for a mini-cartridge in a cross-flow filtration mode (52.5 and 298.4mgg(-1) dry weight for PEVA-TREN-Cu(II) and PEVA-IDA-Cu(II), respectively) was somewhat higher than the equilibrium capacity (9.2 and 73.3mgg(-1) dry weight for PEVA-TREN-Cu(II) and PEVA-IDA-Cu(II), respectively). When mini-cartridges were used, the dynamic adsorption capacity of IDA-Cu(II) was the same for both mini-cartridge and agarose gel. Copyright (c) 2013 John Wiley & Sons, Ltd.
URI: http://repositorio.unifesp.br/handle/11600/36790
ISSN: 0952-3499
Other Identifiers: http://dx.doi.org/10.1002/jmr.2296
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