Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/36677
Title: Evaluation of passage of fetal erythrocytes into maternal circulation after invasive obstetric procedures
Authors: Meleti, Daniela [UNIFESP]
De Oliveira, Leandro Gustavo [UNIFESP]
Araujo Junior, Edward [UNIFESP]
Rabachini Caetano, Ana Carolina [UNIFESP]
Boute, Tatiane [UNIFESP]
Machado Nardozza, Luciano Marcondes [UNIFESP]
Moron, Antonio Fernandes [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Keywords: fetomaternal hemorrhage
flow cytometry
invasive obstetric procedures
Kleihauer-Betke test
-fetoprotein
Issue Date: 1-Sep-2013
Publisher: Wiley-Blackwell
Citation: Journal of Obstetrics and Gynaecology Research. Hoboken: Wiley-Blackwell, v. 39, n. 9, p. 1374-1382, 2013.
Abstract: Aim: the aim of this study was to evaluate the passage of fetal erythrocytes into the maternal circulation after invasive obstetric procedures, using the Kleihauer-Betke test, flow cytometry and -fetoprotein concentration in maternal blood.Material and Methods: This was a prospective descriptive study on patients who underwent: amniocentesis, cordocentesis, chorionic villus sample, amniotic infusion, bladder drainage and ventricular-amniotic shunt to investigate the karyotype; treatment for hydrocephalus, oligohydramnios, obstructive uropathy and polyhydramnios; and investigation of lung maturity. Maternal blood samples were collected before and 60min after the invasive obstetric procedure in order to evaluate the passage of fetal erythrocytes using the Kleihauer-Betke test, flow cytometry and -fetoprotein concentration.Results: in total, 43 invasive obstetric procedures were performed. the procedures performed were: 27 cases of amniocentesis (62.7%), seven cases of cordocentesis (16.2%), four chorionic villus samples (9.4%), two amniotic infusions (4.7%), two ventricular-amniotic shunts and one bladder drainage (2.3%). After one case of cordocentesis with two puncture attempts via the placenta, a significant increase in fetal erythrocytes was detected using the three methods. After another cordocentesis with one puncture via the placenta, a significant increase in fetal erythrocytes was detected using flow cytometry and -fetoprotein concentration, but not through the Kleihauer-Betke test. the other 41 samples did not show any significant increase in fetal erythrocytes in the maternal blood.Conclusion: Invasive obstetric procedures performed during prenatal care are safe when performed by experienced professionals with the proper technique, with minimal chance of passage of fetal erythrocytes into the maternal compartment.
URI: http://repositorio.unifesp.br/handle/11600/36677
ISSN: 1341-8076
Other Identifiers: http://dx.doi.org/10.1111/jog.12073
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