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Title: CXCL12 N-terminal end is sufficient to induce chemotaxis and proliferation of neural stem/progenitor cells
Authors: Filippo, Thais Raquel Martins [UNIFESP]
Galindo, Layla Testa [UNIFESP]
Barnabé, Gabriela Filoso [UNIFESP]
Ariza, Carolina Batista [UNIFESP]
Mello, Luiz Eugenio Araujo de Moraes [UNIFESP]
Juliano, Maria Aparecida [UNIFESP]
Juliano, Luiz [UNIFESP]
Porcionatto, Marimélia Aparecida [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Issue Date: 1-Sep-2013
Publisher: Elsevier B.V.
Citation: Stem Cell Research. Amsterdam: Elsevier B.V., v. 11, n. 2, p. 913-925, 2013.
Abstract: Neural stem/progenitor cells (NSC) respond to injury after brain injuries secreting IL-1, IL-6, TNF-alpha, IL-4 and IL-10, as well as chemokine members of the CC and CXC ligand families. CXCL12 is one of the chemokines secreted at an injury site and is known to attract NSC-derived neuroblasts, cells that express CXCL12 receptor, CXCR4. Activation of CXCR4 by CXCL12 depends on two domains located at the N-terminal of the chemokine. in the present work we aimed to investigate if the N-terminal end of CXCL12, where CXCR4 binding and activation domains are located, was sufficient to induce NSC-derived neuroblast chemotaxis. Our data show that a synthetic peptide analogous to the first 21 amino acids of the N-terminal end of CXCL12, named PepC-C (KPVSLSYRCPCRFFESHIARA), is able to promote chemotaxis of neuroblasts in vivo, and stimulate chemotaxis and proliferation of CXCR4+ cells in vitro, without affecting NSC fate. We also show that PepC-C upregulates CXCL12 expression in vivo and in vitro. We suggest the N-terminal end of CXCL12 is responsible for a positive feedback loop to maintain a gradient of CXCL12 that attracts neuroblasts from the subventricular zone into an injury site. (C) 2013 the Authors. Published by Elsevier B.V. All rights reserved.
ISSN: 1873-5061
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