Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/36443
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dc.contributor.authorFranciozi, Carlos Eduardo da Silveira [UNIFESP]
dc.contributor.authorTarini, Victor Alexandre Ferreira [UNIFESP]
dc.contributor.authorReginato, Rejane Daniele [UNIFESP]
dc.contributor.authorGonçalves, Patrícia dos Reis Sousa [UNIFESP]
dc.contributor.authorMedeiros, Valquíria Pereira de [UNIFESP]
dc.contributor.authorFerretti, Mario [UNIFESP]
dc.contributor.authorDreyfuss, Juliana Luporini [UNIFESP]
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.contributor.authorFaloppa, Flávio [UNIFESP]
dc.date.accessioned2016-01-24T14:31:54Z-
dc.date.available2016-01-24T14:31:54Z-
dc.date.issued2013-07-01
dc.identifierhttp://dx.doi.org/10.1016/j.joca.2013.04.007
dc.identifier.citationOsteoarthritis and Cartilage. Oxford: Elsevier B.V., v. 21, n. 7, p. 965-972, 2013.
dc.identifier.issn1063-4584
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36443-
dc.description.abstractObjective: To investigate the hypothesis that strenuous running is a predisposing factor for osteoarthritis.Design: Wistar rats were divided into two groups: a control group (CG) and a trained group (TG). the TG underwent a strenuous treadmill running training regimen of controlled intensity, exhibiting progressively improvement of fitness over 12 weeks, running at least 55 km during this period and finally performing an ultra-endurance running exercise to exhaustion. After this period, rats from both groups were euthanized and their knees removed. the articular cartilage was dissected and submitted to histomorphometrical, histomorphological, and immunohistochemical analyses evaluating cell death pathway (caspase-3 and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick 'end labeling (TUNEL)) and inflammatory cytokines [interleukin-1 alpha (IL-1 alpha) and tumor necrosis factor-alpha (TNF-alpha)]. in addition, the tissues were analyzed regarding the types and the content of glycosaminoglycans.Results: the TG knee joints exhibited increase in the number of chondrocytes and chondrocyte clusters, as well as significantly increased levels of caspase-3, a protein involved in apoptosis, and of inflammatory cytokines IL-1 alpha and TNF-alpha. in addition, histologically higher grades of osteoarthritis (Osteoarthritis Research Society International - OARSI grading), and significantly decreased levels of chondroitin sulfate and hyaluronic acid. Knee cartilage thickness and TUNEL did not significantly differ between the two groups.Conclusions: the articular cartilage of rats subjected to a strenuous running regimen of controlled intensity exhibited molecular and histological characteristics that are present in osteoarthritis. (C) 2013 Osteoarthritis Research Society International. Published by Elsevier B.V. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent965-972
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofOsteoarthritis and Cartilage
dc.rightsAcesso aberto
dc.subjectArticular cartilageen
dc.subjectExtracellular matrixen
dc.subjectApoptosisen
dc.subjectRunningen
dc.subjectChondroitin sulfateen
dc.subjectHyaluronic aciden
dc.titleGradual strenuous running regimen predisposes to osteoarthritis due to cartilage cell death and altered levels of glycosaminoglycansen
dc.typeArtigo
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Orthopaed & Traumatol, BR-04038032 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Morphol & Genet, BR-04038032 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Program Mol Biol, BR-04038032 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Orthopaed & Traumatol, BR-04038032 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Morphol & Genet, BR-04038032 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Program Mol Biol, BR-04038032 São Paulo, Brazil
dc.identifier.fileWOS000321882900012.pdf
dc.identifier.doi10.1016/j.joca.2013.04.007
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000321882900012
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