Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/36278
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dc.contributor.authorSalinas-Souza, Carolina [UNIFESP]
dc.contributor.authorOliveira, Indhira Dias [UNIFESP]
dc.contributor.authorOliveira, Renato de [UNIFESP]
dc.contributor.authorSeixas Alves, Maria Teresa de [UNIFESP]
dc.contributor.authorPetrilli, Antonio Sergio [UNIFESP]
dc.contributor.authorToledo, Silvia Regina Caminada de [UNIFESP]
dc.date.accessioned2016-01-24T14:31:41Z
dc.date.available2016-01-24T14:31:41Z
dc.date.issued2013-05-01
dc.identifierhttp://dx.doi.org/10.1007/s10616-012-9487-5
dc.identifier.citationCytotechnology. Dordrecht: Springer, v. 65, n. 3, p. 347-353, 2013.
dc.identifier.issn0920-9069
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36278
dc.description.abstractOsteosarcoma (OS) is the most frequent malignant bone tumour in children and adolescents. in metastatic patients, the most common site of metastasis is the lung. There are relatively few cell lines of metastatic OS reported in the literature and the cytogenetic aspects of OS metastases are still controversial and inconclusive. Here we describe the establishment of a new OS cell line, M-OS, from a pulmonary metastasis of a typical osteoblastic OS of an 11-year-old boy with metastatic OS at diagnosis. M-OS cells have been maintained in culture for over 50 passages for more than 1 year. M-OS was characterized by immunohistochemistry, conventional cytogenetics and fluorescence in situ hybridization (FISH). in order to evaluate in vitro cell modification, the immunohistochemical analysis was performed in three different moments of the cell line: 10th, 30th and 50th passages. the conventional cytogenetic analysis revealed the ploidy of M-OS cell line as near-diploid, with most metaphases hyperdiploid and tetraploid. We found a copy number gain of MDM2 gene as the most frequent alteration in the FISH analysis. the immunohistochemical analysis confirmed that M-OS cell line maintained the osteogenic nature even after all passages for the cell line establishment in vitro.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipGrupo de Apoio ao Adolescente e a Crianca com Cancer (GRAACC/UNIFESP)
dc.format.extent347-353
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofCytotechnology
dc.rightsAcesso restrito
dc.subjectOsteosarcomaen
dc.subjectLung metastasisen
dc.subjectCell lineen
dc.subjectCytogeneticsen
dc.subjectFISHen
dc.titleEstablishment and cytogenetic characterization of a cell line from a pulmonary metastasis of osteosarcomaen
dc.typeArtigo
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniversidade Federal de São Paulo, Pediat Oncol Inst IOP GRAACC UNIFESP, Dept Pediat, Genet Lab, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Morphol & Genet, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Thorac Surg, BR-04024002 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Pathol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Pediat Oncol Inst IOP GRAACC UNIFESP, Dept Pediat, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Pediat Oncol Inst IOP GRAACC UNIFESP, Dept Pediat, Genet Lab, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Morphol & Genet, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Thorac Surg, BR-04024002 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Pathol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Pediat Oncol Inst IOP GRAACC UNIFESP, Dept Pediat, BR-04023062 São Paulo, Brazil
dc.identifier.doi10.1007/s10616-012-9487-5
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000316220500004
Appears in Collections:Artigo

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