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Title: Predicting the Proteins of Angomonas deanei, Strigomonas culicis and Their Respective Endosymbionts Reveals New Aspects of the Trypanosomatidae Family
Authors: Motta, Maria Cristina Machado
Azevedo Martins, Allan Cezar de
Souza, Silvana Sant'Anna de
Costa Catta-Preta, Carolina Moura
Silva, Rosane
Klein, Cecilia Coimbra
Paula de Almeida, Luiz Gonzaga
Cunha, Oberdan de Lima
Ciapina, Luciane Prioli
Brocchi, Marcelo
Colabardini, Ana Cristina
Lima, Bruna de Araujo
Machado, Carlos Renato
Almeida Soares, Celia Maria de
Probst, Christian Macagnan
Afonso de Menezes, Claudia Beatriz
Thompson, Claudia Elizabeth
Bartholomeu, Daniella Castanheira
Gradia, Daniela Fiori
Pavoni, Daniela Parada
Grisard, Edmundo C.
Fantinatti-Garboggini, Fabiana
Marchini, Fabricio Klerynton
Rodrigues-Luiz, Gabriela Flaia
Wagner, Glauber
Goldman, Gustavo Henrique
Rangel Fietto, Juliana Lopes
Elias, Maria Carolina
Goldman, Maria Helena S.
Sagot, Marie-France
Pereira, Maristela
Stoco, Patricia H.
Mendonca-Neto, Rondon Pessoa de
Ribeiro Teixeira, Santuza Maria
Ferreira Maciel, Talles Eduardo
Oliveira Mendes, Tiago Antonio de
Uermenyi, Turan P.
Souza, Wanderley de
Schenkman, Sergio [UNIFESP]
Ribeiro de Vasconcelos, Ana Tereza
Universidade Federal do Rio de Janeiro (UFRJ)
Lab Bioinformat
INRIA Grenoble Rhone Alpes
Univ Lyon 1
Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Lab Nacl Ciencia & Tecnol Bioetano
Universidade Federal de Minas Gerais (UFMG)
Universidade Federal de Goiás (UFG)
Fundacao Oswaldo Cruz
Universidade Federal de Santa Catarina (UFSC)
Universidade Federal de Viçosa (UFV)
Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Issue Date: 3-Apr-2013
Publisher: Public Library Science
Citation: Plos One. San Francisco: Public Library Science, v. 8, n. 4, 20 p., 2013.
Abstract: Endosymbiont-bearing trypanosomatids have been considered excellent models for the study of cell evolution because the host protozoan co-evolves with an intracellular bacterium in a mutualistic relationship. Such protozoa inhabit a single invertebrate host during their entire life cycle and exhibit special characteristics that group them in a particular phylogenetic cluster of the Trypanosomatidae family, thus classified as monoxenics. in an effort to better understand such symbiotic association, we used DNA pyrosequencing and a reference-guided assembly to generate reads that predicted 16,960 and 12,162 open reading frames (ORFs) in two symbiont-bearing trypanosomatids, Angomonas deanei (previously named as Crithidia deanei) and Strigomonas culicis (first known as Blastocrithidia culicis), respectively. Identification of each ORF was based primarily on TriTrypDB using tblastn, and each ORF was confirmed by employing getorf from EMBOSS and Newbler 2.6 when necessary. the monoxenic organisms revealed conserved housekeeping functions when compared to other trypanosomatids, especially compared with Leishmania major. However, major differences were found in ORFs corresponding to the cytoskeleton, the kinetoplast, and the paraflagellar structure. the monoxenic organisms also contain a large number of genes for cytosolic calpain-like and surface gp63 metalloproteases and a reduced number of compartmentalized cysteine proteases in comparison to other TriTryp organisms, reflecting adaptations to the presence of the symbiont. the assembled bacterial endosymbiont sequences exhibit a high A+T content with a total of 787 and 769 ORFs for the Angomonas deanei and Strigomonas culicis endosymbionts, respectively, and indicate that these organisms hold a common ancestor related to the Alcaligenaceae family. Importantly, both symbionts contain enzymes that complement essential host cell biosynthetic pathways, such as those for amino acid, lipid and purine/pyrimidine metabolism. These findings increase our understanding of the intricate symbiotic relationship between the bacterium and the trypanosomatid host and provide clues to better understand eukaryotic cell evolution.
ISSN: 1932-6203
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