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Title: Study of Tryptophan Lifetime Fluorescence Following Low-Density Lipoprotein Modification
Authors: Sicchieri, Leticia Bonfante
Monteiro, Andrea Moreira
Samad, Ricardo Elgul
Ito, Amando Siuiti
Figueiredo Neto, Antonio Martins
Vieira, Nilson Dias
Gidlund, Magnus
Courrol, Lilia Coronato [UNIFESP]
Comissao Nacl Energia Nucl
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Keywords: Low-density lipoprotein (LDL)
Time-resolved fluorescence
Ultra-short pulsed laser
Issue Date: 1-Apr-2013
Publisher: Soc Applied Spectroscopy
Citation: Applied Spectroscopy. Frederick: Soc Applied Spectroscopy, v. 67, n. 4, p. 379-384, 2013.
Abstract: In this paper we report the effects of the irradiation of low-density lipoprotein (LDL) by ultra-short laser pulses to obtain in vitro alterations mimicking proatherogenic modifications occurring in vivo in LDL. the modifications by metallic ions (copper and iron) and ultra-short laser pulses were studied by fluorescence steady state and time-resolved lifetime measurements. the results demonstrate that the modifications caused by ultra-short laser pulses and by iron affect the tryptophan residues of apolipoprotein B-100 (Apo-B), slightly decreasing fluorescent lifetimes, with almost no modifications in pre-exponential factors, indicating preservation of structural properties around the fluorophore. On the other hand, oxidation by copper strongly affects the Apo-B protein associated with LDL. We describe a fast, inexpensive, and nondestructive fluorescence-based method that is readily accessible to provide the LDL particle characterization.
ISSN: 0003-7028
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Appears in Collections:Em verificação - Geral

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