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Title: Autoantibodies to 60 kDa SS-A/Ro yield a specific nuclear myriad discrete fine speckled immunofluorescence pattern
Authors: Dellavance, Alessandra
Alvarenga, Rossana Rassi [UNIFESP]
Rodrigues, Silvia Helena [UNIFESP]
Barbosa, Silvia Helena
Pinto Camilo, Amandia Cristina
Okamoto Shiguedomi, Herika Santiago
Rodrigues, Silvia Sanchez
Silva, Cristiane Gallindo
Coelho Andrade, Luis Eduardo [UNIFESP]
Fleury Med & Hlth Labs
Universidade Federal de São Paulo (UNIFESP)
Keywords: Antinuclear antibodies
Systemic lupus erythematosus
Sjogren's syndrome
Issue Date: 1-Apr-2013
Publisher: Elsevier B.V.
Citation: Journal of Immunological Methods. Amsterdam: Elsevier B.V., v. 390, n. 1-2, p. 35-40, 2013.
Abstract: Objectives: To report on a novel immunofluorescence pattern specifically associated with antibodies to SS-A/Ro.Methods: A novel immunofluorescence pattern, herein designated SS-A/Ro pattern, was characterized as myriad discrete fine speckles throughout the nucleus. Eighty-six sequential samples presenting the SS-A/Ro pattern and 64 samples presenting non-SS-A/Ro nuclear fine speckled pattern at the ANA-HEp-2 routine were screened for SS-A/Ro reactivity. Conversely, 48 samples with known reactivity to 60 kDa SS-A/Ro, 13 samples with exclusive reactivity to 52 kDa SS-A/Ro, and 48 SS-A/Ro-negative samples were analyzed for the ANA-HEp-2 pattern.Results: Eighty-five of the 86 samples (98.8%) presenting the SS-A/Ro pattern and 15 of the 64 (23.4%) samples with non-SS-A/Ro fine speckled pattern reacted with 60 kDa SS-A/Ro. Conversely, 44 (91.6%) of 48 samples with known reactivity to 60 kDa SS-A/Ro presented the SS-A/Ro pattern and four (8.4%) presented non-SS-A/Ro fine speckled pattern. None of the 48 anti-SS-A/Ro-negative samples and none of anti-52 kDa SS-A/Ro-positive samples yielded the SS-A/Ro pattern. This immunofluorescence pattern was observed in different commercial HEp-2 cell slides and in homemade HEp-2 cell slides.Conclusions: the SS-A/Ro pattern belongs to the group of immunofluorescence patterns that hold strong association with the respective autoantibody specificities, such as those associated with CENP-F and NuMA-1. the identification of the SS-A/Ro pattern at the ANA-HEp-2 screening routine shall lead to specific tests for the identification of anti-SS-A/Ro antibodies. (C) 2013 Elsevier B.V. All rights reserved.
ISSN: 0022-1759
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