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|Title:||The Efficacy of L. (L.) chagasi Excreted-Secreted Antigens (ESAs) for Visceral Leishmaniasis Diagnosis Is Due To Low Levels of Cross-Reactivity|
Barbiéri, Clara Lucia [UNIFESP]
Lindoso, Jose Angelo Lauletta
Umezawa, Eufrosina Setsu
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Inst Emilio Ribas São Paulo
|Publisher:||Amer Soc Trop Med & Hygiene|
|Citation:||American Journal of Tropical Medicine and Hygiene. Mclean: Amer Soc Trop Med & Hygiene, v. 88, n. 3, p. 559-565, 2013.|
|Abstract:||The analysis of promastigote excreted-secreted antigen (ESA) reactivity with 53 visceral leishmaniasis (VL) cases showed that each sample reacted regardless of the antigen or the Leishmania species used in enzyme-linked immunosorbent assay (ELISA) displayed 100% positivity with the L. (L.) chagasi ESA-blot recognizing bands of molecular weight ranging from 26.5 to 31.5 kDa. the analysis of 160 non-visceral cases showed that 5% of the samples cross-reacted with the L. (L.) chagasi ESA-ELISA and 9.4% reacted with the ESA isolated from L. (L.) amazonensis and L. (V.) braziliensis, whereas a high cross-reaction ranging from 24.4% to 25% was observed with total crude promastigote antigens (PRO-ELISA). the ESA-blot of L. (L.) chagasi tested with non-visceral sera samples showed a cross-reaction with 8.8% of cases; most of these cases represented tegumentary leishmaniasis and only one acute chagasic case. These data lead us to recommend the use of ESA as an alternative antigen in VL diagnosis.|
|Appears in Collections:||Em verificação - Geral|
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