Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/36014
Title: The Efficacy of L. (L.) chagasi Excreted-Secreted Antigens (ESAs) for Visceral Leishmaniasis Diagnosis Is Due To Low Levels of Cross-Reactivity
Authors: Pinedo-Cancino, Viviana
Kesper, Norival
Barbiéri, Clara Lucia [UNIFESP]
Lindoso, Jose Angelo Lauletta
Umezawa, Eufrosina Setsu
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Inst Emilio Ribas São Paulo
Issue Date: 1-Mar-2013
Publisher: Amer Soc Trop Med & Hygiene
Citation: American Journal of Tropical Medicine and Hygiene. Mclean: Amer Soc Trop Med & Hygiene, v. 88, n. 3, p. 559-565, 2013.
Abstract: The analysis of promastigote excreted-secreted antigen (ESA) reactivity with 53 visceral leishmaniasis (VL) cases showed that each sample reacted regardless of the antigen or the Leishmania species used in enzyme-linked immunosorbent assay (ELISA) displayed 100% positivity with the L. (L.) chagasi ESA-blot recognizing bands of molecular weight ranging from 26.5 to 31.5 kDa. the analysis of 160 non-visceral cases showed that 5% of the samples cross-reacted with the L. (L.) chagasi ESA-ELISA and 9.4% reacted with the ESA isolated from L. (L.) amazonensis and L. (V.) braziliensis, whereas a high cross-reaction ranging from 24.4% to 25% was observed with total crude promastigote antigens (PRO-ELISA). the ESA-blot of L. (L.) chagasi tested with non-visceral sera samples showed a cross-reaction with 8.8% of cases; most of these cases represented tegumentary leishmaniasis and only one acute chagasic case. These data lead us to recommend the use of ESA as an alternative antigen in VL diagnosis.
URI: http://repositorio.unifesp.br/handle/11600/36014
ISSN: 0002-9637
Other Identifiers: http://dx.doi.org/10.4269/ajtmh.12-0587
Appears in Collections:Em verificação - Geral

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