Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/35943
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dc.contributor.authorMartin, Fabio O. [UNIFESP]
dc.contributor.authorMenezes, Sidneia S. de
dc.contributor.authorChiba, Akemi K. [UNIFESP]
dc.contributor.authorLanghi, Dante M.
dc.contributor.authorNardozza, Luciano M. M. [UNIFESP]
dc.contributor.authorChiattone, Carlos S.
dc.contributor.authorBordin, Jose O. [UNIFESP]
dc.date.accessioned2016-01-24T14:31:14Z-
dc.date.available2016-01-24T14:31:14Z-
dc.date.issued2013-02-01
dc.identifierhttp://dx.doi.org/10.1016/j.transci.2012.09.004
dc.identifier.citationTransfusion and Apheresis Science. Oxford: Pergamon-Elsevier B.V., v. 48, n. 1, p. 113-116, 2013.
dc.identifier.issn1473-0502
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35943-
dc.description.abstractBackground: the D-negative phenotype is the result of the total RHD gene deletion in almost all Caucasians, but it accounts for only about 20% in Africans and 70% in Asians. in Africans the RHD Psi that is one of the most important causes of the D-negative phenotype. We investigated the RHD polymorphisms in D-negative phenotype mixed Brazilians who have anti-D alloantibody.Study design and methods: Blood samples from 130 individuals previously typed as D-negative were phenotyped again using: (a) two tube reagents (Anti-D blend reagent, Cellular line TH-28, MS-26; and Anti-D polyclonal); (b) one gel test ID-Card for Rh subgroups including C-w and K antigen; and (c) ABO/Rh (Anti-D blend reagent, Cellular line 175-2, LDM3). the method used for RHD screening detected the presence of RHD exon 10 and intron 4. Sequence analysis was performed on PCR products amplified from genomic DNA for all 10 exons RHD gene.Results: We found that 1181130 (90.8%) of D-negative tested individuals had total RHD gene deletion, while 12/130 (9.2%) showed RHD gene polymorphisms. the RHD Psi was found in 10 (7.7%) individuals, one sample (0.77%) hybrid RHD-CE-D-s /RHD Psi, and another (0.77%) weak D type 4.2.Conclusions: the results showed that the RHD gene was present in 9.2% of racially mixed Brazilians who produced usually clinically significant anti-D alloantibodies. Therefore, the data showed that careful attention is necessary for clinicians in applying RhD genotyping to transfusion medicine in populations with high rate of racial admixture. (C) 2012 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent113-116
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTransfusion and Apheresis Science
dc.rightsAcesso restrito
dc.subjectRHD geneen
dc.subjectPolymorphismsen
dc.subjectAnti-D alloantibodyen
dc.titleRHD gene polymorphisms in alloimmunized RhD-negative individuals with high rate of racial admixtureen
dc.typeArtigo
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFac Ciencias Med Santa Casa São Paulo
dc.description.affiliationUniversidade Federal de São Paulo, Disciplina Hematol & Hemoterapia, São Paulo, Brazil
dc.description.affiliationFac Ciencias Med Santa Casa São Paulo, Disciplina Hematol & Hemoterapia, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Disciplina Ginecol & Obstet, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Disciplina Hematol & Hemoterapia, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Disciplina Ginecol & Obstet, São Paulo, Brazil
dc.description.sponsorshipIDFAPESP: 05/55.237-9
dc.identifier.doi10.1016/j.transci.2012.09.004
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000316646900020
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