Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/35830
Title: POSTERIOR HYALOID DETACHMENT and INTERNAL LIMITING MEMBRANE PEELING ASSISTED BY ANTHOCYANINS FROM ACAI FRUIT (EUTERPE OLERACEA) and 10 OTHER NATURAL VITAL DYES Experimental Study in Cadaveric Eyes
Authors: Chen, Jane [UNIFESP]
Ferreira, Magno Antonio [UNIFESP]
Farah, Michel Eid [UNIFESP]
Carvalho, Andre Maia de [UNIFESP]
Alves Ferreira, Raquel Eustaquio
Moraes Filho, Milton Nunes de [UNIFESP]
Souza Lima-Filho, Acacio Alves
Lago, Joao Henrique G. [UNIFESP]
Sartorelli, Patricia [UNIFESP]
Rodrigues, Eduardo Buchele [UNIFESP]
Ferreira, Eber
Peris, Cristiane [UNIFESP]
Maia, Mauricio [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Uberlândia (UFU)
Ophthalmos
Ethno
Keywords: chromovitrectomy
natural dyes
posterior vitreous detachment
internal limiting membrane peeling
Issue Date: 1-Jan-2013
Publisher: Lippincott Williams & Wilkins
Citation: Retina-the Journal of Retinal and Vitreous Diseases. Philadelphia: Lippincott Williams & Wilkins, v. 33, n. 1, p. 89-96, 2013.
Abstract: Purpose: the purpose of this study was to determine whether natural dyes facilitate posterior hyaloid detachment (posterior vitreous detachment [PVD]) and retinal internal limiting membrane (ILM) peeling in human eyes.Methods: Open-sky vitrectomy with posterior hyaloid and ILM removal was performed in 86 human cadaveric eyes. After core vitrectomy, 11 different dyes were injected into the vitreous cavity to aid hyaloid detachment and ILM removal. the dyes were allowed to settle on the macula for 5 minutes after PVD and were removed by mechanical aspiration. Intraocular forceps were used for ILM peeling, which was confirmed by light microscopy of the peeled tissue. Acai fruit (Euterpe oleracea) extract and 10 additional dyes from plants or animal sources were tested: pomegranate (Punica granatum), logwood (Haematoxylum campechianum), chlorophyll extract from alfalfa (Medicago sativa), cochineal (Dactylopius coccus), hibiscus (Hibiscus rosa-sinensis), indigo (Indigofera tinctoria), paprika (Capiscum annuum), turmeric (Curcuma longa), old fustic (Maclura tinctoria), and grape (Vitis vinifera).Results: the dyes facilitated PVD and ILM peeling. Acai fruit (E. oleracea) extract, logwood (H. campechianum), cochineal (D. coccus), and old fustic (M. tinctoria) facilitated PVD in all cases; dye-assisted PVD was compared with triamcinolone-assisted PVD performed previously in a comparative model. Acai fruit (E. oleracea) extract, cochineal (D. coccus), and chlorophyll extract from alfalfa (M. sativa) showed the best capability for ILM staining; dye-assisted ILM removal was compared with the ILM peeling guided by indocyanine green staining performed previously in a comparative model. Light microscopy confirmed the ILM removal in all cases.Conclusion: Anthocyanin dye of the acai fruit (E. oleracea) and the dyes from cochineal (D. coccus) and chlorophyll extract from alfalfa (M. sativa) resulted in the best capability for posterior hyaloid and ILM staining in human cadaveric eyes and may be a useful tool for vitreoretinal surgery. RETINA 33:89-96, 2013
URI: http://repositorio.unifesp.br/handle/11600/35830
ISSN: 0275-004X
Other Identifiers: http://dx.doi.org/10.1097/IAE.0b013e3182618a6d
Appears in Collections:Em verificação - Geral

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