Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/35790
Title: Biocontrol activity of Bacillus against a GFP-marked Pseudomonas syringae pv. tomato on tomato phylloplane
Authors: Lanna Filho, R.
Souza, R. M. de
Ferreira, A.
Quecine, M. C. [UNIFESP]
Alves, Eduardo
Azevedo, J. L. de [UNIFESP]
Univ Fed Rio Grande do Sul
Universidade Federal de Lavras (UFLA)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Federal de São Paulo (UNIFESP)
Keywords: Bacterial speck
Biological control
Endophytic bacteria
Bacillus pumilus
Bacillus amyloliquefaciens
Green fluorescent protein
Issue Date: 1-Jan-2013
Publisher: Springer
Citation: Australasian Plant Pathology. Dordrecht: Springer, v. 42, n. 6, p. 643-651, 2013.
Abstract: We report the biocontrol activity of the endophytic bacteria Bacillus pumilus and Bacillus amyloliquefacies against the plant pathogenic bacterium Pseudomonas syringae pv. tomato strain NS4 transformed with the GFP expressing gene. P. s. pv. tomato strain NS4 was obtained from the transformation of P. s. pv. tomato wild-type strain NW with the plasmid pNKGFP containing GFP-cassette for chromosomal integration. the GFP-marked strain was tested for hypersensitivity and pathogenicity, as well as population studies on the phylloplane, to determine its epidemiology and survival. in all of the bioassays strain NS4 presented similar characteristics to the wild-type, and was hence chosen as the model strain for these studies with antagonistic endophytic bacterial strains. in the biocontrol experiments, tomato plants were pre-inoculated with the endophytic bacteria 4 days prior to inoculation with P. s. pv. tomato strains. On the tomato phylloplane the P. s. pv. tomato (strains NW and NS4) populations were drastically reduced, and tomato leaves showed reduced numbers of bacterial speck lesions, comparable to the standard chemical treatment copper oxychloride. Additionally, under epifluorescence microscopy, few GFP-tagged cells of strain NS4 were observed colonizing important niches on the tomato phylloplane. However, leaves untreated with the antagonists presented a large number of GFP-tagged cell aggregates. Our results demonstrated that endophytic bacteria can also act efficiently on the biocontrol of bacterial speck when applied as a foliar spray on the leaves. in addition, we highlighted the use of GFP-marked strain NS4 as a model system to study biocontrol agent and pathogen interactions, and growth and development of the pathogen on the tomato leaf surface.
URI: http://repositorio.unifesp.br/handle/11600/35790
ISSN: 0815-3191
Other Identifiers: http://dx.doi.org/10.1007/s13313-013-0233-z
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