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Title: Low-Level Laser Therapy Decreases Renal Interstitial Fibrosis
Authors: Mayrink Oliveira, Fabiana Aparecida
Meneghin Moraes, Ana Carolina
Paiva, Amanda Povoa
Schinzel, Vania
Correa-Costa, Matheus [UNIFESP]
Semedo, Patricia [UNIFESP]
Castoldi, Angela [UNIFESP]
Cenedeze, Marcos Antonio [UNIFESP]
Fortes Oliveira, Roberto Sotto-Maior
Bastos, Marcus Gomes
Saraiva Camara, Niels Olsen [UNIFESP]
Sanders-Pinheiro, Helady
Univ Fed Juiz de Fora
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Issue Date: 1-Dec-2012
Publisher: Mary Ann Liebert Inc
Citation: Photomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc, v. 30, n. 12, p. 705-713, 2012.
Abstract: Objective: the purpose of this study was to investigate the effect of low-level laser therapy (LLLT) on chronic kidney disease (CKD) in a model of unilateral ureteral obstruction (UUO). Background data: Regardless of the etiology, CKD involves progressive widespread tissue fibrosis, tubular atrophy, and loss of kidney function. This process also occurs in kidney allograft. At present, effective therapies for this condition are lacking. We investigated the effects of LLLT on the interstitial fibrosis that occurs after experimental UUO in rats. Methods: the occluded kidney of half of the 32 Wistar rats that underwent UUO received a single intraoperative dose of LLLT (AlGaAs laser, 780 nm, 22.5 J/cm(2), 30mW, 0.75W/cm(2), 30 sec on each of nine points). After 14 days, renal fibrosis was assessed by Sirius red staining under polarized light. Immunohistochemical analyses quantitated the renal tissue cells that expressed fibroblast (FSP-1) and myofibroblast (alpha-SMA) markers. Reverse transcriptase polymerase chain reaction (RT-PCR) was performed to determine the mRNA expression of interleukin (IL)-6, monocyte chemotactic protein-1 (MCP-1), transforming growth factor (TGF)-beta 1 and Smad3. Results: the UUO and LLLT animals had less fibrosis than the UUO animals, as well having decreased expression inflammatory and pro-fibrotic markers. Conclusions: for the first time, we showed that LLLT had a protective effect regarding renal interstitial fibrosis. It is conceivable that by attenuating inflammation, LLLT can prevent tubular activation and transdifferentiation, which are the two processes that mainly drive the renal fibrosis of the UUO model.
ISSN: 1549-5418
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Appears in Collections:Artigo

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