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Title: SORL1 and SIRT1 mRNA expression and promoter methylation levels in aging and Alzheimer's Disease
Authors: Furuya, Tatiane Katsue [UNIFESP]
Oliveira da Silva, Patricia Natalia [UNIFESP]
Payão, Spencer Luiz Marques [UNIFESP]
Rasmussen, Lucas Trevizani
Labio, Roger Willian de
Bertolucci, Paulo Henrique Ferreira [UNIFESP]
Braga, Ianna Lacerda Sampaio [UNIFESP]
Chen, Elizabeth Suchi [UNIFESP]
Turecki, Gustavo
Mechawar, Naguib
Mill, Jonathan
Smith, Marilia de Arruda Cardoso [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Fac Med Marilia FAMEMA
McGill Univ
Kings Coll London
Universidade de São Paulo (USP)
Keywords: Alzheimer's Disease
SORL1 and SIRT1 mRNA expression
Peripheral blood leukocytes
Post mortem brain tissue
Promoter DNA methylation
Issue Date: 1-Dec-2012
Publisher: Elsevier B.V.
Citation: Neurochemistry International. Oxford: Pergamon-Elsevier B.V., v. 61, n. 7, p. 973-975, 2012.
Abstract: Alzheimer's Disease (AD) is a neurodegenerative disorder and the most common cause of dementia among the elderly. Efforts have been made to understand the genetic and epigenetic mechanisms involved in the development of this disease. As SORL1 (sortilin-related receptor) and SIRT1 (sirtuin I) genes have been linked to AD pathogenesis, we aimed to investigate their mRNA expression and promoter DNA methylation in post mortem brain tissues (entorhinal and auditory cortices and hippocampus) from healthy elderly subjects and AD patients. We also evaluated these levels in peripheral blood leukocytes from young, healthy elderly and AD patients, investigating whether there was an effect of age on these profiles. the comparative CT method by Real Time PCR and MALDI-TOF mass spectrometry were used to analyze gene expression and DNA methylation, respectively. SORL1 gene was differently expressed in the peripheral blood leukocytes and might act as a marker of aging in this tissue Furthermore, we found that SORL1 promoter DNA methylation might act as one of the mechanisms responsible for the differences in expression observed between blood and brain for both healthy elderly and AD patients groups. the impact of these studied genes on AD pathogenesis remains to be better clarified. (C) 2012 Elsevier B.V. All rights reserved.
ISSN: 0197-0186
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