Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/35559
Title: Leishmanicidal, antiproteolytic and antioxidant evaluation of natural biflavonoids isolated from Garcinia brasiliensis and their semisynthetic derivatives
Authors: Gontijo, Vanessa Silva
Judice, Wagner A. S.
Codonho, Barbara
Pereira, Ivan Oliveira
Assis, Diego Magno [UNIFESP]
Januario, Jaqueline Pereira
Caroselli, Elide E. [UNIFESP]
Juliano, Maria Aparecida [UNIFESP]
Dosatti, Amanda de Carvalho
Marques, Marcos Jose
Viegas Junior, Claudio
Santos, Marcelo Henrique dos
Univ Fed Alfenas
Mogi das Crazes Univ
Univ Vale Rio Verde
Universidade Federal de São Paulo (UNIFESP)
Keywords: Garcinia brasiliensis
Biflavonoids
Natural products
Semisynthesis
Antioxidants
Antiproteolytic
Leishmania amazonensis
Issue Date: 1-Dec-2012
Publisher: Elsevier B.V.
Citation: European Journal of Medicinal Chemistry. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 58, p. 613-623, 2012.
Abstract: The natural biflavonoids morelloflavone-4'-O-beta-D-glycosyl (1), (+/-)-fukugiside (2) and morelloflavone (3) were isolated from the ethyl acetate extract (EAEE) of dried and powdered fruit epicarps of Garcinia brasiliensis and derivatives of morelloflavone were semi-synthesised. Morelloflavone-7,4',7 '',3',4'-penta-O-acetyl (4), morelloflavone-7,4',7 '',3',4'-penta-O-methyl (5) and morelloflavone-7,4',7 '',3',4'-penta-O-butanoyl (6) were prepared by acylation and alkylation reactions. All compounds showed leishmanicidal, antiproteolytic and antioxidant activities in addition to exhibiting low cytotoxicity. Compounds 4, 5 and 6 were highly active against Leishmania amazonensis promastigote forms compared to natural compounds of low lipophilicity, exhibiting IC50 values of 0.0147, 0.0403 and 0.0189 mu M, respectively. Compounds 4, 5 and 6 were also highly active against amastigote forms with IC50 values of 0.042, 0.0603 and 0.059 mu M, respectively. in addition, highly inhibitory activity against r-CPB2.8 and r-CPB3 isoforms was observed with these compounds. Notably, compounds 3 and 4 were the most active against r-CPB2.8 with IC50 values of 0.4200 and 0.6744 mu M, respectively. Compounds 5 and 6 also showed significant inhibitory activities with very similar IC50 values of 1.2663 and 1.0122, respectively. However, compounds 1 and 2 exhibited the lowest inhibitory activity against r-CPB2.8, almost 6 and 11-fold less active than the natural compound 3. in L. (L.) amazonensis lysates, and compounds 3 and 6 were the most active inhibitors of amastigote lysates at pH 5, which is near the pH environment of the parasitophorous vacuole within the macrophage. Finally, compounds 1, 2 and 3 exhibited effective antioxidant activity compared to the reference antioxidant ascorbic acid. However, the activity was lower than that of butylhydroxytoluene (BHT), which may be related to the reduced number of phenolic hydroxyl groups that were replaced by more lipophilic substituents in derivatives 4-6. Compounds 4-6 exhibited reduced antioxidant activity as evidenced by their higher EC50 values. These results provide new perspectives on drug development for the treatment of leishmaniasis and inhibitory enzyme activity on Leishmania (L.) mexicana cysteine proteases and other isoforms. (C) 2012 Published by Elsevier Masson SAS.
URI: http://repositorio.unifesp.br/handle/11600/35559
ISSN: 0223-5234
Other Identifiers: http://dx.doi.org/10.1016/j.ejmech.2012.06.021
Appears in Collections:Em verificação - Geral

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