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Title: Cytogenetic biomonitoring of peripheral blood and oral mucosa cells from car painters
Authors: Silva, Victor Hugo Pereira da [UNIFESP]
Moura, Carolina Foot Gomes de [UNIFESP]
Spadari-Bratfisch, Regina Celia [UNIFESP]
Ribeiro, Daniel Araki [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Keywords: Buccal mucosa cells
Tongue cells
Car painters
Micronucleus test
Comet assay
Issue Date: 1-Sep-2012
Publisher: Informa Healthcare
Citation: Toxicology Mechanisms and Methods. London: Informa Healthcare, v. 22, n. 7, p. 497-501, 2012.
Abstract: The aim of the present study was to comparatively evaluate genomic damage and cellular death in exfoliated oral mucosa cells and peripheral blood from car painters. A total of 24 car painters and 19 healthy controls (non-exposed individuals) were included in this setting. Individuals had epithelial cells from cheek mucosa (left and right side) mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the specific nuclear phenotypes. A total of 5 mu L from peripheral blood was collected for the single cell gel (comet) assay. the results pointed out statistically significant differences (p < 0.05) of micronucleated oral mucosa cells from car painters. in addition, DNA damage was detected in peripheral blood cells by single cell gel (comet) assay. Nevertheless, exposure to car paints did not cause increases other nuclear alterations closely related to cytotoxicity such as karrhyorexis, pyknosis and karyolysis in buccal mucosa cells. in summary, the results of the present study suggest that car painters comprise a high risk group since paints can induce genotoxic and mutagenic effects in peripheral blood and oral mucosa cells, respectively.
ISSN: 1537-6516
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