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Title: Combined fish oil and astaxanthin supplementation modulates rat lymphocyte function
Authors: Otton, Rosemari
Marin, Douglas Popp
Bolin, Anaysa Paola
Santos Macedo, Rita de Cassia
Campoio, Thais Regina
Fineto, Claudio
Guerra, Beatriz Alves
Leite, Jose Roberto [UNIFESP]
Barros, Marcelo Paes
Mattei, Rita [UNIFESP]
Univ Cruzeiro Sul
Universidade Federal de São Paulo (UNIFESP)
Keywords: Antioxidant
Fish oil
Oxidative stress
n-3 fatty acids
Issue Date: 1-Sep-2012
Publisher: Springer
Citation: European Journal of Nutrition. Heidelberg: Springer Heidelberg, v. 51, n. 6, p. 707-718, 2012.
Abstract: Higher intakes of n-3 polyunsaturated fatty acids that are abundant in marine fishes have been long described as a good nutritional intervention with increasing clinical benefits to cardiovascular health, inflammation, mental, and neurodegenerative diseases. the present study was designed to investigate the effect of daily fish oil (FO-10 mg EPA/kg body weight (BW) and 7 mg DHA/kg BW) intake by oral gavage associated with the antioxidant astaxanthin (ASTA-1 mg/kg BW) on the redox metabolism and the functional properties of lymphocytes from rat lymph nodes.This study was conducted by measurements of lymphocyte proliferation capacity, ROS production [superoxide (O (2) (aEuro cent a') ) and hydrogen peroxide (H2O2)], nitric oxide (NOaEuro cent) generation, intracellular calcium release, oxidative damage to lipids and proteins, activities of major antioxidant enzymes, GSH/GSSG content, and cytokines release.After 45 days of FO + ASTA supplementation, the proliferation capacity of activated T- and B-lymphocytes was significantly diminished followed by lower levels of O (2) (aEuro cent a') , H2O2 and NOaEuro cent production, and increased activities of total/SOD, GR and GPx, and calcium release in cytosol. ASTA was able to prevent oxidative modification in cell structures through the suppression of the oxidative stress condition imposed by FO. l-selectin was increased by FO, and IL-1 beta was decreased only by ASTA supplementation.We can propose that association of ASTA with FO could be a good strategy to prevent oxidative stress induced by polyunsaturated fatty acids and also to potentiate immuno-modulatory effects of FO.
ISSN: 1436-6207
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