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Title: Synthesis and properties of cyclic gomesin and analogues
Authors: Machado, Alessandra
Fazio, Marcos A. [UNIFESP]
Miranda, Antonio [UNIFESP]
Daffre, Sirlei
Teresa Machini, M.
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Univ Tecnol Fed Parana
Univ Prebiteriana Mackenzie
Keywords: antimicrobial peptide
head-to-tail cyclization
peptide truncation
high temperatures
Issue Date: 1-Sep-2012
Publisher: Wiley-Blackwell
Citation: Journal of Peptide Science. Hoboken: Wiley-Blackwell, v. 18, n. 9, p. 588-598, 2012.
Abstract: Gomesin (Gm) was the first antimicrobial peptide (AMP) isolated from the hemocytes of a spider, the Brazilian mygalomorph Acanthoscurria gomesiana. We have been studying the properties of this interesting AMP, which also displays anticancer, antimalarial, anticryptococcal and anti-Leishmania activities. in the present study, the total syntheses of backbone-cyclized analogues of Gm (two disulfide bonds), [Cys(Acm)2,15]-Gm (one disulfide bond) and [Thr2,6,11,15,d-Pro9]-Gm (no disulfide bonds) were accomplished, and the impact of cyclization on their properties was examined. the consequence of simultaneous deletion of pGlu1 and Arg16-Glu-Arg18-NH2 on Gm antimicrobial activity and structure was also analyzed. the results obtained showed that the synthetic route that includes peptide backbone cyclization on resin was advantageous and that a combination of 20% DMSO/NMP, EDC/HOBt, 60?degrees C and conventional heating appears to be particularly suitable for backbone cyclization of bioactive peptides. the biological properties of the Gm analogues clearly revealed that the N-terminal amino acid pGlu1 and the amidated C-terminal tripeptide Arg16-Glu-Arg18-NH2 play a major role in the interaction of Gm with the target membranes. Moreover, backbone cyclization practically did not affect the stability of the peptides in human serum; it also did not affect or enhanced hemolytic activity, but induced selectivity and, in some cases, discrete enhancements of antimicrobial activity and salt tolerance. Because of its high therapeutic index, easy synthesis and lower cost, the [Thr2,6,11,15,d-Pro9]-Gm analogue remains the best active Gm-derived AMP developed so far; nevertheless, its elevated instability in human serum may limit its therapeutic potential. Copyright (c) 2012 European Peptide Society and John Wiley & Sons, Ltd.
ISSN: 1075-2617
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