Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/35176
Title: Giant Amazonian fish pirarucu (Arapaima gigas): Its viscera as a source of thermostable trypsin
Authors: Freitas-Junior, Augusto C. V.
Costa, Helane M. S.
Icimoto, Marcelo Yudi [UNIFESP]
Hirata, Izaura Yoshico [UNIFESP]
Marcondes, Marcelo [UNIFESP]
Carvalho, Luiz B.
Oliveira, Vitor [UNIFESP]
Bezerra, Ranilson S.
Universidade Federal de Pernambuco (UFPE)
Universidade Federal de São Paulo (UNIFESP)
Keywords: Arapaima gigas
Air-breathing fish
Fish processing waste
Digestive enzymes
Proteases
Trypsin purification
Issue Date: 15-Aug-2012
Publisher: Elsevier B.V.
Citation: Food Chemistry. Oxford: Elsevier B.V., v. 133, n. 4, p. 1596-1602, 2012.
Abstract: A trypsin was purified from pyloric caeca of pirarucu (Arapaima gigas). the effect of metal ions and protease inhibitors on its activity and its physicochemical and kinetic properties, as well its N-terminal sequence, were determined. A single band (28.0 kDa) was observed by SDS-PAGE. Optimum pH and temperature were 9.0 and 65 degrees C, respectively. the enzyme was stable after incubation for 30 min in a wide pH range (6.0-11.5) and at 55 degrees C. the kinetic parameters K-m, k(cat) and k(cat)/K-m were 0.47 +/- 0.042 mM, 1.33 s(-1) and 2.82 s(-1) mM(-1), respectively, using BApNA as substrate. This activity was shown to be very sensitive to some metal ions, such as Fe2+, Hg2+, Zn2+, Al3+, Pb2+, and was highly inhibited by trypsin inhibitors. the trypsin N-terminal sequence IVGGYECPRNSVPYQ was found. the features of this alkaline peptidase suggest that it may have potential for industrial applications (e.g. food and detergent industries). (C) 2012 Elsevier B.V. All rights reserved.
URI: http://repositorio.unifesp.br/handle/11600/35176
ISSN: 0308-8146
Other Identifiers: http://dx.doi.org/10.1016/j.foodchem.2012.02.056
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