Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/35000
Title: Lipidomic Analysis of Extracellular Vesicles from the Pathogenic Phase of Paracoccidioides brasiliensis
Authors: Vallejo, Milene C. [UNIFESP]
Nakayasu, Ernesto S.
Longo, Larissa V. G. [UNIFESP]
Ganiko, Luciane
Lopes, Felipe G.
Matsuo, Alisson L. [UNIFESP]
Almeida, Igor C.
Puccia, Rosana [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Univ Texas El Paso
Issue Date: 22-Jun-2012
Publisher: Public Library Science
Citation: Plos One. San Francisco: Public Library Science, v. 7, n. 6, 10 p., 2012.
Abstract: Background: Fungal extracellular vesicles are able to cross the cell wall and transport molecules that help in nutrient acquisition, cell defense, and modulation of the host defense machinery.Methodology/Principal Findings: Here we present a detailed lipidomic analysis of extracellular vesicles released by Paracoccidioides brasiliensis at the yeast pathogenic phase. We compared data of two representative isolates, Pb3 and Pb18, which have distinct virulence profiles and phylogenetic background. Vesicle lipids were fractionated into different classes and analyzed by either electrospray ionization- or gas chromatography-mass spectrometry. We found two species of monohexosylceramide and 33 phospholipid species, including phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid, phosphatidylserine, phosphatidylinositol, and phosphatidylglycerol. Among the phospholipid-bound fatty acids in extracellular vesicles, C181 predominated in Pb3, whereas C18:2 prevailed in Pb18. the prevalent sterol in Pb3 and Pb18 vesicles was brassicasterol, followed by ergosterol and lanosterol. Inter-isolate differences in sterol composition were observed, and also between extracellular vesicles and whole cells.Conclusions/Significance: the extensive lipidomic analysis of extracellular vesicles from two P. brasiliensis isolates will help to understand the composition of these fungal components/organelles and will hopefully be useful to study their biogenesis and role in host-pathogen interactions.
URI: http://repositorio.unifesp.br/handle/11600/35000
ISSN: 1932-6203
Other Identifiers: http://dx.doi.org/10.1371/journal.pone.0039463
Appears in Collections:Artigo

Files in This Item:
File Description SizeFormat 
WOS000305730900064.pdf714.6 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.