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Title: Cytogenetic instability of dental pulp stem cell lines
Authors: Duailibi, Monica Talarico [UNIFESP]
Kulikowski, Leslie Domenici
Duailibi, Silvio Eduardo [UNIFESP]
Nunes Lipay, Monica Vannucci [UNIFESP]
Melaragno, Maria Isabel
Ferreira, Lydia Masako [UNIFESP]
Vacanti, Joseph Phillip
Yelick, Pamela Crotty
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
INCT Biofabris
Massachusetts Gen Hosp
Harvard Univ
Dept Surg
Tufts Univ
Keywords: Chromosomal abnormalities
Cell transplantation
Tooth tissue engineering
Human dental stem cells
Issue Date: 1-Feb-2012
Publisher: Springer
Citation: Journal of Molecular Histology. Dordrecht: Springer, v. 43, n. 1, p. 89-94, 2012.
Abstract: Human adult stem cells (hASCs) offer a potentially renewable source of cell types that are easily isolated and rapidly expanded for use in regenerative medicine and cell therapies without the complicating ethical problems that are associated with embryonic stem cells. However, the eventual therapeutic use of hASCs requires that these cells and their derivatives maintain their genomic stability. There is currently a lack of systematic studies that are aimed at characterising aberrant chromosomal changes in cultured ASCs over time. However, the presence of mosaicism and accumulation of karyotypic abnormalities within cultured cell subpopulations have been reported. To investigate cytogenetic integrity of cultured human dental stem cell (hDSC) lines, we analysed four expanded hDSC cultures using classical G banding and fluorescent in situ hybridisation (FISH) with X chromosome specific probe. Our preliminary results revealed that about 70% of the cells exhibited karyotypic abnormalities including polyploidy, aneuploidy and ring chromosomes. the heterogeneous spectrum of abnormalities indicates a high frequency of chromosomal mutations that continuously arise upon extended culture. These findings emphasise the need for the careful analysis of the cytogenetic stability of cultured hDSCs before they can be used in clinical therapies.
ISSN: 1567-2379
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