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|Title:||A Novel Approach for Subretinal Implantation of Ultrathin Substrates Containing Stem Cell-Derived Retinal Pigment Epithelium Monolayer|
Diniz, Bruno [UNIFESP]
Thomas, Padmaja B.
Ahuja, Ashish K.
Hinton, David R.
Hikita, Sherry T.
Johnson, Lincoln V.
Clegg, Dennis O.
Thomas, Biju B.
Humayun, Mark S.
Univ So Calif
Univ Calif Santa Barbara
Chang Gung Mem Hosp
Hosp Evangel Curitiba
Universidade Federal de São Paulo (UNIFESP)
Retinal pigment epithelium
Royal College of Surgeon rats
|Citation:||Ophthalmic Research. Basel: Karger, v. 48, n. 4, p. 186-191, 2012.|
|Abstract:||Objective: To evaluate the feasibility of a new technique for the implantation of ultrathin substrates containing stem cell-derived retinal pigment epithelium (RPE) cells into the subretinal space of retina-degenerate Royal College of Surgeon (RCS) rats. Methods: A platform device was used for the implantation of 4-mu m-thick parylene substrates containing a monolayer of human embryonic stem cell-derived RPE (hESC-RPE). Normal Copenhagen rats (n = 6) and RCS rats (n = 5) were used for the study. Spectral-domain optical coherence tomography (SD-OCT) scanning and histological examinations were performed to confirm placement location of the implant. hESC- RPE cells attached to the substrate before and after implantation were evaluated using standard cell counting techniques. Results: SD-OCT scanning and histological examination revealed that the substrates were precisely placed in the rat's subretinal space. the hESC-RPE cell monolayer that covered the surface of the substrate was found to be intact after implantation. Cell counting data showed that less than 2% of cells were lost from the substrate due to the implantation procedure (preimplantation count 2,792 +/- 74.09 cells versus postimplantation count 2,741 +/- 62.08 cells). Detailed microscopic examination suggested that the cell loss occurred mostly along the edges of the implant. Conclusion: With the help of this platform device, it is possible to implant ultrathin substrates containing an RPE monolayer into the rat's subretinal space. This technique can be a useful approach for stem cell-based tissue bioengineering techniques in retinal transplantation research. Copyright (c) 2012 S. Karger AG, Basel|
|Appears in Collections:||Em verificação - Geral|
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