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Title: Neuronal differentiation involves a shift from glucose oxidation to fermentation
Authors: Fornazari, Maynara
Nascimento, Isis C. [UNIFESP]
Nery, Arthur A.
Caldeira da Silva, Camille C.
Kowaltowski, Alicia J.
Ulrich, Henning
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Keywords: Neuronal differentiation
Fermentative glucose metabolism
Mitochondrial uncoupling
P19 embryonal carcinoma cells
E14Tg2A embryonic stem cells
Issue Date: 1-Oct-2011
Publisher: Springer
Citation: Journal of Bioenergetics and Biomembranes. New York: Springer/plenum Publishers, v. 43, n. 5, p. 531-539, 2011.
Abstract: Energy metabolism in the adult brain consumes large quantities of glucose, but little is known to date regarding how glucose metabolism changes during neuronal differentiation, a process that is highly demanding energetically. We studied changes in glucose metabolism during neuronal differentiation of P19 mouse embryonal carcinoma cells, E14Tg2A embryonic stem cells as well as during brain development of BLC57 mice. in all these models, we find that neurogenesis is accompanied by a shift from oxidative to fermentative glucose metabolism. This shift is accompanied by both a decrease in mitochondrial enzymatic activities and mitochondrial uncoupling. in keeping with this finding, we also observe that differentiation does not require oxidative metabolism, as indicated by experiments demonstrating that the process is preserved in cells treated with the ATP synthase inhibitor oligomycin. Overall, we provide evidence that neuronal differentiation involves a shift from oxidative to fermentative metabolism, and that oxidative phosphorylation is not essential for this process.
ISSN: 0145-479X
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Appears in Collections:Em verificação - Geral

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