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Title: A Novel beta-Glucosidase from Sporidiobolus pararoseus: Characterization and Application in Winemaking
Authors: Baffi, Milla Alves
Tobal, Thaise
Lago, Joao Henrique G. [UNIFESP]
Leite, Rodrigo S. R.
Boscolo, Mauricio
Gomes, Eleni
Da-Silva, Roberto
Universidade Federal de Uberlândia (UFU)
Universidade Federal de São Paulo (UNIFESP)
Univ Fed Grande Dourados UFGD
São Paulo State Univ UNESP
Keywords: aroma
Sporidiobolus pararoseus
Issue Date: 1-Sep-2011
Publisher: Wiley-Blackwell
Citation: Journal of Food Science. Malden: Wiley-Blackwell, v. 76, n. 7, p. C997-C1002, 2011.
Abstract: For the first time, the production of an extracellular beta-glucosidase (Sp-beta-gl) by a Sporidiobolus pararoseus yeast strain is reported. the Sp-beta-gl activity was quantified, characterized, and assessed for its efficiency in releasing aroma-enhancing compounds in wines. the maximum enzymatic synthesis was after 72 h of growth in a complex media with 20 g/L of cellobiose. the optimal pH and temperature were 5.5 and at 50 degrees C, respectively. It showed a wide range of pH stability and exhibited quite high thermostability at low temperatures. in addition, this beta-glucosidase revealed tolerance to wine-associated inhibitory compounds (sugars and ethanol), showing suitable characteristics for all the stages of alcoholic fermentation. the hydrolysis of the glycosidic terpenes by Sp-beta-gl was studied by gas chromatography, and its ability to efficiently release free terpenols has been demonstrated. the concentrations of geraniol, linalool, alpha-terpineol, and nerol were significantly increased in treated wines. These results suggest the potential application of this new yeast beta-glucosidase as an aroma-enhancing enzyme in winemaking.
ISSN: 0022-1147
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