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Title: Tooth Tissue Engineering: Optimal Dental Stem Cell Harvest Based on Tooth Development
Authors: Duailibi, Monica Talarico [UNIFESP]
Duailibi, Silvio Eduardo [UNIFESP]
Duailibi Neto, Eduardo Felippe
Negreiros, Renata Matalon
Jorge, Waldyr Antonio
Ferreira, Lydia Masako [UNIFESP]
Vacanti, Joseph Phillip
Yelick, Pamela Crotty
Tufts Univ
Universidade Federal de São Paulo (UNIFESP)
Natl Inst Sci & Technol
Universidade de São Paulo (USP)
Massachusetts Gen Hosp
Dept Surg
Harvard Univ
Keywords: Tooth development
Tooth tissue engineering
Human dental stem cells
Human tooth development
Tooth rapid prototyping
Issue Date: 1-Jul-2011
Publisher: Wiley-Blackwell
Citation: Artificial Organs. Malden: Wiley-Blackwell, v. 35, n. 7, p. E129-E135, 2011.
Abstract: Our long-term objective is to devise reliable methods to generate biological replacement teeth exhibiting the physical properties and functions of naturally formed human teeth. Previously, we demonstrated the successful use of tissue engineering approaches to generate small, bioengineered tooth crowns from harvested pig and rat postnatal dental stem cells (DSCs). To facilitate characterizations of human DSCs, we have developed a novel radiographic staging system to accurately correlate human third molar tooth developmental stage with anticipated harvested DSC yield. Our results demonstrated that DSC yields were higher in less developed teeth (Stages 1 and 2), and lower in more developed teeth (Stages 3, 4, and 5). the greatest cell yields and colony-forming units (CFUs) capability was obtained from Stages 1 and 2 tooth dental pulp. We conclude that radiographic developmental staging can be used to accurately assess the utility of harvested human teeth for future dental tissue engineering applications.
ISSN: 0160-564X
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Appears in Collections:Artigo

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