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Title: Leucurogin, a new recombinant disintegrin cloned from Bothrops leucurus (white-tailed-jararaca) with potent activity upon platelet aggregation and tumor growth
Authors: Higuchi, D. A.
Almeida, M. C.
Barros, C. C.
Sanchez, E. F.
Pesquero, P. R.
Lang, E. A. S.
Samaan, M.
Araujo, Ronaldo Carvalho [UNIFESP]
Pesquero, João Bosco [UNIFESP]
Pesquero, J. L.
Universidade Federal de Minas Gerais (UFMG)
Univ Mogi das Cruzes
Proteobras Desenvolvimento Biotecnol Ltda
Fundacao Ezequiel Dias
Universidade Federal de São Paulo (UNIFESP)
Keywords: Disintegrins
Bothrops leucurus
Platelet aggregation
Tumor growth
Issue Date: 1-Jul-2011
Publisher: Elsevier B.V.
Citation: Toxicon. Oxford: Pergamon-Elsevier B.V., v. 58, n. 1, p. 123-129, 2011.
Abstract: Disintegrins and disintegrins-like proteins are able to inhibit platelet aggregation and integrin-mediated cell adhesion. the aim of this study was to produce one disintegrin-like cloned from Bothrops leucurus venom gland and to characterize it regarding biological activity. the recombinant protein was purified by one step procedure involving anion-exchange chromatography (DEAE-cellulose) and presented a molecular mass of 10.4 kDa. the purified protein was able to inhibit platelet aggregation induced by collagen (IC(50) = 0.65 mu M) and to inhibit growth of Ehrlich tumor implanted in mice by more than 50% after 7 days administration of 10 mu g/day. No effects were observed upon adenosine 5'diphosphate (ADP)-and arachidonic acid (AA)-induced platelet aggregation. the recombinant protein was recognized by an antibody specific for jararhagin one metalloproteinase isolated from Bothrops jararaca venom, and therefore it was named leucurogin. Anti-angiogenesis effect of leucurogin was evaluated by the sponge implant model. After 7 days administration leucurogin inhibited, in a dose dependent way, the vascularization process in the sponge. Leucurogin represents a new biotechnological tool to understand biological processes where disintegrins-like are involved and may help to characterize integrins that can be involved in development and progression of malignant cells. (C) 2011 Elsevier B.V. All rights reserved.
ISSN: 0041-0101
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