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dc.contributor.authorNakahata, Adriana Miti [UNIFESP]
dc.contributor.authorMayer, Barbara
dc.contributor.authorRies, Christian
dc.contributor.authorAndrade de Paula, Claudia Alessandra [UNIFESP]
dc.contributor.authorKarow, Marisa
dc.contributor.authorNeth, Peter
dc.contributor.authorSampaio, Misako U. [UNIFESP]
dc.contributor.authorJochum, Marianne
dc.contributor.authorOliva, Maria Luiza V. [UNIFESP]
dc.identifier.citationBiological Chemistry. Berlin: Walter de Gruyter & Co, v. 392, n. 4, p. 327-336, 2011.
dc.description.abstractSupplementary to the efficient inhibition of trypsin, chymotrypsin, plasma kallikrein, and plasmin already described by the EcTI inhibitor from Enterolobium contortisiliquum, it also blocks human neutrophil elastase (K(iapp)=4.3 nM) and prevents phorbol ester (PMA)-stimulated activation of matrix metalloproteinase (MMP)-2 probably via interference with membrane-type 1 (MT1)-MMP. Moreover, plasminogen-induced activation of proMMP-9 and processing of active MMP-2 was also inhibited. Furthermore, the effect of EcTI on the human cancer cell lines HCT116 and HT29 (colorectal), SkBr-3 and MCF-7 (breast), K562 and THP-1 (leukemia), as well as on human primary fibroblasts and human mesenchymal stem cells (hMSCs) was studied. EcTI inhibited in a concentration range of 1.0-2.5 mu M rather specifically tumor cell viability without targeting primary fibroblasts and hMSCs. Taken together, our data indicate that the polyspecific proteinase inhibitor EcTI prevents proMMP activation and is cytotoxic against tumor cells without affecting normal tissue remodeling fibroblasts or regenerative hMSCs being an important tool in the studies of tumor cell development and dissemination.en
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.publisherWalter de Gruyter & Co
dc.relation.ispartofBiological Chemistry
dc.rightsAcesso restrito
dc.subjectKunitz inhibitorsen
dc.subjectmatrix metalloproteinasesen
dc.subjectmesenchymal stem cellsen
dc.subjectplant proteinasesen
dc.titleThe effects of a plant proteinase inhibitor from Enterolobium contortisiliquum on human tumor cell linesen
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Munich
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv Munich, Dept Surg, Clin Grosshadern, D-81377 Munich, Germany
dc.description.affiliationUniv Munich, Div Clin Chem & Clin Biochem, D-80366 Munich, Germany
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
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