Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/33575
Title: Development and Evaluation of a Latex Agglutination Test for the Serodiagnosis of Paracoccidioidomycosis
Authors: Silveira-Gomes, Fabiola
Sarmento, Dayse Nogueira
Pinto, Thifany Mendes
Pimentel, Rosiane Ferreira
Nepomuceno, Livia Barreto
Espirito Santo, Elaine Patricia T.
Mesquita-da-Costa, Maurimelia
Camargo, Zoilo Pires [UNIFESP]
Marques-da-Silva, Silvia Helena
Fed Univ Para
Universidade Federal de São Paulo (UNIFESP)
Evandro Chagas Inst
Issue Date: 1-Apr-2011
Publisher: Amer Soc Microbiology
Citation: Clinical and Vaccine Immunology. Washington: Amer Soc Microbiology, v. 18, n. 4, p. 604-608, 2011.
Abstract: Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin America. It is caused by the dimorphic fungus Paracoccidioides brasiliensis. the immunodiffusion (ID) test is one of the most widely used techniques for PCM serologic diagnosis due to the simplicity and low costs of its execution. However, it requires trained and qualified people to execute it. the purpose of this study was to evaluate a latex particle agglutination (LA) test for the detection of anti-P. brasiliensis antibodies by using pooled crude exoantigens from the fungus. Fifty-one serum samples obtained from patients with PCM were tested. Positivity was observed in 84% (43/51) of these patients, and the agglutination patterns varied from small clumps with a cloudy background to large clumps with a clear background. the antibody titer reactivity ranged from 1:2 to 1:64. Cross-reactivity was observed in sera from patients with aspergillosis, histoplasmosis, and nonfungal disease. Serum samples obtained from healthy donors were not reactive. the sensitivity and specificity of the LA test were 84% and 81%, respectively. When comparing the LA test with the double-immunodiffusion test, we found an agreement of 92%. Further work is needed to improve the performance of the LA assay before it can be proposed as a reliable diagnostic tool, mainly in laboratories with little infrastructure.
URI: http://repositorio.unifesp.br/handle/11600/33575
ISSN: 1556-6811
Other Identifiers: http://dx.doi.org/10.1128/CVI.00130-10
Appears in Collections:Artigo

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