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Title: A New Approach for Heparin Standardization: Combination of Scanning UV Spectroscopy, Nuclear Magnetic Resonance and Principal Component Analysis
Authors: Lima, Marcelo A. [UNIFESP]
Rudd, Timothy R.
Farias, Eduardo H. C. de [UNIFESP]
Ebner, Lyvia F. [UNIFESP]
Gesteira, Tarsis F. [UNIFESP]
Souza, Lauro M. de
Mendes, Aline [UNIFESP]
Córdula, Carolina Ribeiro [UNIFESP]
Martins, Joao R. M. [UNIFESP]
Hoppensteadt, Debra
Fareed, Jawed
Sassaki, Guilherme L.
Yates, Edwin A.
Tersariol, Ivarne Luis dos Santos [UNIFESP]
Nader, Helena Bonciani [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Univ Liverpool
Loyola Univ
Univ Fed Parana
Issue Date: 18-Jan-2011
Publisher: Public Library Science
Citation: Plos One. San Francisco: Public Library Science, v. 6, n. 1, 9 p., 2011.
Abstract: The year 2007 was marked by widespread adverse clinical responses to heparin use, leading to a global recall of potentially affected heparin batches in 2008. Several analytical methods have since been developed to detect impurities in heparin preparations; however, many are costly and dependent on instrumentation with only limited accessibility. A method based on a simple UV-scanning assay, combined with principal component analysis (PCA), was developed to detect impurities, such as glycosaminoglycans, other complex polysaccharides and aromatic compounds, in heparin preparations. Results were confirmed by NMR spectroscopy. This approach provides an additional, sensitive tool to determine heparin purity and safety, even when NMR spectroscopy failed, requiring only standard laboratory equipment and computing facilities.
ISSN: 1932-6203
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