Please use this identifier to cite or link to this item: http://repositorio.unifesp.br/handle/11600/33110
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dc.contributor.authorMatsuo, Alisson L. [UNIFESP]
dc.contributor.authorTanaka, Aparecida S. [UNIFESP]
dc.contributor.authorJuliano, Maria A. [UNIFESP]
dc.contributor.authorRodrigues, Elaine G. [UNIFESP]
dc.contributor.authorTravassos, Luiz R. [UNIFESP]
dc.date.accessioned2016-01-24T14:05:43Z-
dc.date.available2016-01-24T14:05:43Z-
dc.date.issued2010-12-01
dc.identifierhttp://dx.doi.org/10.1007/s00109-010-0671-9
dc.identifier.citationJournal of Molecular Medicine-jmm. New York: Springer, v. 88, n. 12, p. 1255-1264, 2010.
dc.identifier.issn0946-2716
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33110-
dc.description.abstractPeptide display on the phage surface has been widely used to identify specific peptides targeting several in vivo and in vitro tumor cells and the tumor vasculature, playing a role in the discovery of bioactive antitumor agents. Bioactive peptides have been selected to target important tumor receptors or apoptosis-associated molecules such as p53. Presently, we attempted to identify potentially antitumor bioactive molecules using the whole cell surface as the recognizable static matrix. Such methodology could be advantageous in cancer therapy because it does not require previous characterization of target molecules. Using a C7C phage display library, we screened for peptides binding to the B16F10-Nex2 melanoma cell surface after pre-absorption on melan-A lineage. After a few rounds of enrichment, 50 phages were randomly selected, amplified, and tested for inhibition of tumor cell proliferation. Seven were active, and the corresponding peptide of each phage was chemically synthesized in the cyclic form and tested in vitro. Three peptides were able to preferentially inhibit the melanoma lineage. A unique peptide, [-CSSRTMHHC-], exhibited in vivo antitumor inhibitory activity against a subcutaneous melanoma challenge, rendering 60% of mice without tumor growth. Further, this peptide also markedly inhibited in vitro and in vivo the tumor cell invasion and cell-to-cell adhesiveness in vitro. This is the first report on a bioactive peptide derived from a C7C library active against whole melanoma cells in vitro and in vivo.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent1255-1264
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofJournal of Molecular Medicine-jmm
dc.rightsAcesso restrito
dc.subjectMelanomaen
dc.subjectPhage displayen
dc.subjectBioactive peptideen
dc.subjectAntitumor activityen
dc.titleA novel melanoma-targeting peptide screened by phage display exhibits antitumor activityen
dc.typeArtigo
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Expt Oncol Unit UNONEX, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Expt Oncol Unit UNONEX, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.identifier.doi10.1007/s00109-010-0671-9
dc.description.sourceWeb of Science
dc.identifier.wosWOS:000284276200008
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