Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/33018
Title: The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
Other Titles: O papel da proteína ligante Fas no estresse oxidativo induzido pelo azoximetano em criptas do colo de ratos
Authors: Bussuan, Luiz Antonio Maksoud [UNIFESP]
Fagundes, Djalma Jose
Marks, Guido
Bussuan, Priscila Maksoud
Teruya, Roberto
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Mato Grosso do Sul (UFMS)
Moreira Salles Inst IMS
Keywords: Fas Ligand Protein
Azoxymethane
Oxidative Stress
Apoptosis
Rats
Issue Date: 1-Nov-2010
Publisher: Acta Cirurgica Brasileira
Citation: Acta Cirurgica Brasileira. São Paulo: Acta Cirurgica Brasileira, v. 25, n. 6, p. 501-506, 2010.
Abstract: Purpose: To study the protein Fas ligand (FasL) on the expression of apoptosis, using a model of oxidative stress induced by azoxymethane (AOM), in the crypt of colon in rats. Methods: Wistar rats (n=14) were assigned into two groups: control (n=7) and AOM (n=7). A single subcutaneous administration of AOM (5mg/kg) or saline solution was performed at the beginning of third week and after three hours samples of proximal colon were collected. the expression of FasL was quantified (Software ImageLab) in percentage of areas in the top, base and all crypt. Results were expressed as mean +/- sd (Shapiro-Wilks test and t Student test) (p <= 0.05). Results: in the animals of CG there was no significant difference between the FasL expression of the top (10.75 +/- 3.33) and basal (11.14 +/- 3.53) colon crypt (p=0.34293740). in the animals of AOM there was no significant difference between the FasL expression of the top (8.86 +/- 4.19) and basal (8.99 +/- 4.08) colon crypt (p=0.78486003). in the animals of CG (10.95 +/- 3.43) and AOM (8.92 +/- 4.13) there was a significant difference of the FasL expression (p=0.026466821). A significantly decrease on the FasL expression was observed in the animals of CG (10.75 +/- 3.33) and AOM (8.86 +/- 4.19) in the top crypt (p=0.00003755*). A significant decrease was also observed in the animals of CG (11.14 +/- 3.53) and AOM (8.99 +/- 4.08) in the basal colon crypt (p=0.00000381**). Conclusion: Azoxymethane induce the oxidative stress and the significantly decrease of FasL expression, although there is no significant difference between basal and top of colon crypt linked to consumption-activation of Fas ligand.
URI: http://repositorio.unifesp.br/handle/11600/33018
ISSN: 0102-8650
Other Identifiers: http://dx.doi.org/10.1590/S0102-86502010000600008
Appears in Collections:Artigo

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.