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Title: ACE2-angiotensin-(1-7)-Mas axis in renal ischaemia/reperfusion injury in rats
Authors: Silveira, Katia D. da
Bosco, Kenia S. Pompermayer
Diniz, Lucio R. L.
Carmona, Adriana Karaoglanovic [UNIFESP]
Cassali, Giovanni D.
Bruna-Romero, Oscar
Sousa, Lirlandia P. de
Teixeira, Mauro M.
Santos, Robson A. S.
Simoes e Silva, Ana C.
Ribeiro Vieira, Maria A.
Universidade Federal de Minas Gerais (UFMG)
Universidade Federal de São Paulo (UNIFESP)
Keywords: acute kidney injury
angiotensin I-converting enzyme (ACE)
angiotensin I-converting enzyme 2 (ACE2)
angiotensin-(1-7) [Ang-(1-7)]
angiotensin II (AngII)
Mas receptor
Issue Date: 1-Nov-2010
Publisher: Portland Press Ltd
Citation: Clinical Science. London: Portland Press Ltd, v. 119, n. 9-10, p. 385-394, 2010.
Abstract: AngII (angiotensin II), ACE (angiotensin I-converting enzyme) and the AT(1) receptor (AngII type I receptor) are associated with the inflammatory process and microvascular dysfunction of AKI (acute kidney injury) induced by renal I/R (ischaemia/reperfusion). However, Ang-(1-7) [angiotensin-(1-7)], ACE2 (angiotensin I-converting enzyme 2) and the Mas receptor also play a role in renal disease models. Therefore, in the present study, we have examined the renal profile of Ang-(1-7), ACE2 and the Mas receptor in renal I/R and compared them with that of AngII, ACE and the AT(1) receptor. Male Wistar rats were submitted to left nephrectomy and ischaemia (45 min) followed by reperfusion (2 or 4 h) in the right kidney. At 4 h of reperfusion, renal AngII was increased (P < 0.01) and renal Ang-(1-7) was decreased substantially (P < 0.05), although plasma levels of both angiotensins were unchanged. in addition, renal I/R decreased the renal mRNA expression of renin (P < 0.05), AT(1) receptors (P < 0.001) and ACE2 (P < 0.05). At 2 and 4 h of reperfusion, renal ACE activity was reduced (P < 0.05). On the other hand, renal expression of the Mas receptor was greatly increased at 4 h of reperfusion (P < 0.01), which was confirmed by immunohistochemical and Western blot analysis. in conclusion, increased renal expression of the Mas receptor associated with changes in the RAS (renin-angiotensin-system)-related peptidases support an important role for the ACE2 Ang-(1-7) Mas axis in AKI.
ISSN: 0143-5221
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