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dc.contributor.authorAndrade, Valeria C. C.
dc.contributor.authorVettore, Andre L. [UNIFESP]
dc.contributor.authorPanepucci, Rodrigo A.
dc.contributor.authorAlmeida, Manuella S. S.
dc.contributor.authorYamamoto, Mihoko
dc.contributor.authorDe Carvalho, Fabricio
dc.contributor.authorCaballero, Otavia L.
dc.contributor.authorZago, Marco Antonio
dc.contributor.authorColleoni, Gisele W. B. [UNIFESP]
dc.identifier.citationLeukemia & Lymphoma. Abingdon: Taylor & Francis Ltd, v. 51, n. 8, p. 1543-1549, 2010.
dc.description.abstractConsidering that the importance of cancer/testis (CT) antigens in multiple myeloma (MM) biology is still under investigation, the present study aimed to: (1) identify genes differentially expressed in MM using microarray analysis of plasma cell samples, separated according to the number of expressed CTs; (2) examine possible pathways related to MM pathogenesis; (3) validate the expression of candidate genes by quantitative real-time PCR (RQ-PCR). Three samples predominantly positive (>6 expressed), including the U266 cell line, and three samples predominantly negative (0 or 1 expressed CT for the 13 analyzed CT antigens), were submitted for microarray analysis. Validation by RQ-PCR from 24 MM samples showed that the ITGAS gene was downregulated in predominantly positive (>6 expressed CTs, p = 0.0030) and in tumor versus normal plasma cells (p = 0.0182). the RhoD gene was overexpressed in tumor plasma cells when compared to normal plasma cells (p = 0.0339). Results of the microarray analysis corroborate the hypothesis that MM could be separated into predominantly positive and predominantly negative expression. the differential expression of ITGA5 and RhoD suggests disruption of the focal adhesion pathway in MM and offers a new target field to be explored in this disease.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofLeukemia & Lymphoma
dc.rightsAcesso restrito
dc.subjectMultiple myelomaen
dc.subjectRhoD geneen
dc.subjectITGA5 geneen
dc.subjectcancer/testis antigensen
dc.titleNumber of expressed cancer/testis antigens identifies focal adhesion pathway genes as possible targets for multiple myeloma therapyen
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionLudwig Inst Canc Res
dc.description.affiliationUniversidade Federal de São Paulo, EPM, Discipline Hematol & Hemotherapy, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biol, Diadema, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, São Paulo, Brazil
dc.description.affiliationLudwig Inst Canc Res, New York Branch, New York, NY USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, EPM, Discipline Hematol & Hemotherapy, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biol, Diadema, Brazil
dc.description.sponsorshipIDFAPESP: 04/13213-3
dc.description.sponsorshipIDFAPESP: 04/12855-1
dc.description.sourceWeb of Science
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