Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/32726
Title: Low mRNA Expression of the Apoptosis-Related Genes CASP3, CASP8, and FAS Is Associated With Low Induction Treatment Response in Childhood Acute Lymphoblastic Leukemia (ALL)
Authors: Mata, Juliana F.
Silveira, Vanessa S.
Mateo, Elvis C.
Cortez, Maria A. A.
Queiroz, Rosane G. P.
Yunes, Jose Andres
Lee, Maria Lucia M. [UNIFESP]
Toledo, Silvia Regina Caminada de [UNIFESP]
Petrilli, Antonio Sergio [UNIFESP]
Brandalise, Silvia R.
Tone, Luiz G.
Scrideli, Carlos A.
Universidade de São Paulo (USP)
Pio XII Fdn Canc Hosp Barretos
Ctr Infantil Boldrini
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Keywords: ALL
apoptosis
children
survival
Issue Date: 15-Jul-2010
Publisher: Wiley-Blackwell
Citation: Pediatric Blood & Cancer. Hoboken: Wiley-liss, v. 55, n. 1, p. 100-107, 2010.
Abstract: Background. Defects in apoptosis signaling have been considered to be responsible for treatment failure in many types of cancer, although with controversial results. the objective of the present study was to assess the expression profile of key apoptosis-related genes in terms of clinical and biological variables and of the survival of children with acute lymphoblastic leukemia (ALL). Procedure. the levels of mRNA expression of the apoptosis-related genes CASP3, CASP8, CASP9, FAS, and BCL2 were analyzed by quantitative real-time PCR in consecutive samples from 139 consecutive children with ALL at diagnosis treated by the Brazilian protocol (GBTLI-ALL 99). Gene expression levels and clinical and biological features were compared by the Mann-Whitney test. Event-free survival (EFS) was calculated by Kaplan-Meier plots and log-rank test. Results. A significant correlation was detected between CASP3, CASP8, CASP9, and FAS expression levels (P<0.01) in ALL samples. Higher levels of BCL2 were significantly associated with white blood cell (WBC) count <50,000/mm(3) at diagnosis (P=0.01) and low risk group classification (P=0.008). Lower expression levels of CASP3, CASP8 and FAS gene were associated with a poor response at day 7 according the GBTLI-ALL 99 protocol (P=0.03, P=0.02 and P=0.008, respectively). There was a relationship between FAS gene expression lower than the 75th percentile and lower 5-year EFS (P=0.02). Conclusion. These findings suggest an association between lower expression levels of the pro-apoptotic genes and a poor response to induction therapy at day 7 and prognosis in childhood ALL. Pediatr Blood Cancer 2010;55:100-107. (C) 2010 Wiley-Liss, Inc.
URI: http://repositorio.unifesp.br/handle/11600/32726
ISSN: 1545-5009
Other Identifiers: http://dx.doi.org/10.1002/pbc.22463
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