Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/32549
Title: Effects of 17 beta-estradiol replacement on the apoptotic effects caused by ovariectomy in the rat hippocampus
Authors: Sales, Sayuri
Ureshino, Rodrigo Portes [UNIFESP]
Santos Pereira, Renato Tavares dos
Amaral Luna, Milene Schmidt
Oliveira, Marcelo Pires de [UNIFESP]
Yamanouye, Norma
Godinho, Rosely Oliveira [UNIFESP]
Smaili, Soraya Soubhi [UNIFESP]
Porto, Catarina Segreti [UNIFESP]
Francis Abdalla, Fernando Mauricio
Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Keywords: Bcl-2
Bax
Hippocampus
17 beta-estradiol
Ovariectomy
Issue Date: 22-May-2010
Publisher: Elsevier B.V.
Citation: Life Sciences. Oxford: Pergamon-Elsevier B.V., v. 86, n. 21-22, p. 832-838, 2010.
Abstract: Aims: the aim of the present study was to investigate the effects of different periods of ovariectomy and 17 beta-estradiol replacement on apoptotic cell death and expression of members of the Bcl-2 family in the rat hippocampus.Main methods: Hippocampi were obtained from rats in proestrus, ovariectomized (15 days, 21 days and 36 days), ovariectomized for 15 days and then treated with 17 beta-estradiol for 7 or 21 days, and rats ovariectomized and immediately treated with 17 beta-estradiol for 21 days. the expression of Bcl-2 and Bax and the number of apoptotic cells were determined.Key findings: Ovariectomy decreased Bcl-2 expression and increased Bax expression and the number of apoptotic cells. Replacement with 17 beta-estradiol (21 days) throughout the post-ovariectomy period reduced the number of apoptotic cells to the control levels, and prevented the effects of ovariectomy on Bax expression, but only partially restored the Bcl-2 expression. After 15 days of ovariectomy. the replacement with 17 beta-estradiol for 21 days, but not for 7 days, restored the Bcl-2 and Bax expression and the percentage of apoptotic cells to the levels found in the proestrus control.Significance: the present results show that a physiological concentration of 17 beta-estradiol may help maintain long-term neuronal viability by regulating the expression of members of the Bcl-2 family. Even after a period of hormonal deprivation, treatment with 17 beta-estradiol is able to restore the expression of Bax and Bcl-2 to control levels, but the duration of the treatment is a key factor to obtain the desired effect. These data provide new understanding into the mechanisms contributing to the neuroprotective action of estrogen. (C) 2010 Elsevier Inc. All rights reserved.
URI: http://repositorio.unifesp.br/handle/11600/32549
ISSN: 0024-3205
Other Identifiers: http://dx.doi.org/10.1016/j.lfs.2010.04.002
Appears in Collections:Em verificação - Geral

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