Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/32383
Title: Altered Function of the SCN1A Voltage-gated Sodium Channel Leads to gamma-Aminobutyric Acid-ergic (GABAergic) Interneuron Abnormalities
Authors: Martin, Melinda S.
Dutt, Karoni
Papale, Ligia Assumpção [UNIFESP]
Dube, Celine M.
Dutton, Stacey B.
Haan, Georgius de
Shankar, Anupama
Tufik, Sergio [UNIFESP]
Meisler, Miriam H.
Baram, Tallie Z.
Goldin, Alan L.
Escayg, Andrew
Univ Calif Irvine
Emory Univ
Universidade Federal de São Paulo (UNIFESP)
Univ Michigan
Issue Date: 26-Mar-2010
Publisher: Amer Soc Biochemistry Molecular Biology Inc
Citation: Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 285, n. 13, p. 9823-9834, 2010.
Abstract: Voltage-gated sodium channels are required for the initiation and propagation of action potentials. Mutations in the neuronal voltage-gated sodium channel SCN1A are associated with a growing number of disorders including generalized epilepsy with febrile seizures plus (GEFS+),(7) severe myoclonic epilepsy of infancy, and familial hemiplegic migraine. To gain insight into the effect of SCN1A mutations on neuronal excitability, we introduced the human GEFS+ mutation SCN1A-R1648H into the orthologous mouse gene. Scn1a(RH/RH) mice homozygous for the R1648H mutation exhibit spontaneous generalized seizures and premature death between P16 and P26, whereas Scn1a(RH/+) heterozygous mice exhibit infrequent spontaneous generalized seizures, reduced threshold and accelerated propagation of febrile seizures, and decreased threshold to flurothyl-induced seizures. Inhibitory cortical interneurons from P5-P15 Scn1a(RH/+) and Scn1a(RH/RH) mice demonstrated slower recovery from inactivation, greater use-dependent inactivation, and reduced action potential firing compared with wild-type cells. Excitatory cortical pyramidal neurons were mostly unaffected. These results suggest that this SCN1A mutation predominantly impairs sodium channel activity in interneurons, leading to decreased inhibition. Decreased inhibition may be a common mechanism underlying clinically distinct SCN1A-derived disorders.
URI: http://repositorio.unifesp.br/handle/11600/32383
ISSN: 0021-9258
Other Identifiers: http://dx.doi.org/10.1074/jbc.M109.078568
Appears in Collections:Em verificação - Geral

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