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Title: Cocaine effects on mouse incentive-learning and human addiction are linked to alpha 2 subunit-containing GABA(A) receptors
Authors: Dixon, Claire I.
Morris, Hannah V.
Breen, Gerome
Desrivieres, Sylvane
Jugurnauth, Sarah
Steiner, Rebecca C.
Vallada, Homero
Guindalini, Camila [UNIFESP]
Laranjeira, Ronaldo [UNIFESP]
Messas, Guilherme [UNIFESP]
Rosahl, Thomas W.
Atack, John R.
Peden, Dianne R.
Belelli, Delia
Lambert, Jeremy J.
King, Sarah L.
Schumann, Gunter
Stephens, David N.
Univ Sussex
Kings Coll London
Universidade de São Paulo (USP)
Merck Sharp & Dohme Ltd
Merck Res Labs
Universidade Federal de São Paulo (UNIFESP)
Univ Dundee
Keywords: GABRA2
behavioral sensitization
nucleus accumbens
mutant mouse
human genetics
Issue Date: 2-Feb-2010
Publisher: Natl Acad Sciences
Citation: Proceedings of the National Academy of Sciences of the United States of America. Washington: Natl Acad Sciences, v. 107, n. 5, p. 2289-2294, 2010.
Abstract: Because GABA(A) receptors containing alpha 2 subunits are highly represented in areas of the brain, such as nucleus accumbens (NAcc), frontal cortex, and amygdala, regions intimately involved in signaling motivation and reward, we hypothesized that manipulations of this receptor subtype would influence processing of rewards. Voltage-clamp recordings from NAcc medium spiny neurons of mice with alpha 2 gene deletion showed reduced synaptic GABA(A) receptor-mediated responses. Behaviorally, the deletion abolished cocaine's ability to potentiate behaviors conditioned to rewards (conditioned reinforcement), and to support behavioral sensitization. in mice with a point mutation in the benzodiazepine binding pocket of alpha 2-GABA(A) receptors (alpha 2H101R), GABAergic neurotransmission in medium spiny neurons was identical to that of WT (i.e., the mutation was silent), but importantly, receptor function was now facilitated by the atypical benzodiazepine Ro 15-4513 (ethyl 8-amido-5,6-dihydro-5-methyl-6-oxo-4H-imidazo [1,5-a] [1,4] benzodiazepine-3-carboxylate). in alpha 2H101R, but not WT mice, Ro 15-4513 administered directly into the NAcc-stimulated locomotor activity, and when given systemically and repeatedly, induced behavioral sensitization. These data indicate that activation of alpha 2-GABA(A) receptors (most likely in NAcc) is both necessary and sufficient for behavioral sensitization. Consistent with a role of these receptors in addiction, we found specific markers and haplotypes of the GABRA2 gene to be associated with human cocaine addiction.
ISSN: 0027-8424
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