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Title: Are endogenous sex hormones related to DNA damage in paradoxically sleep-deprived female rats?
Authors: Andersen, Monica L. [UNIFESP]
Ribeiro, Daniel A. [UNIFESP]
Alvarenga, Tathiana A. [UNIFESP]
Silva, Andressa [UNIFESP]
Araujo, Paula [UNIFESP]
Zager, Adriano [UNIFESP]
Tenorio, Neuli M. [UNIFESP]
Tufik, Sergio [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Keywords: Sleep deprivation
DNA damage
Estrous cycle
Single cell gel (comet) assay
Female rats
Issue Date: 1-Feb-2010
Publisher: Elsevier B.V.
Citation: Hormones and Behavior. San Diego: Academic Press Inc Elsevier Science, v. 57, n. 2, p. 216-221, 2010.
Abstract: The aim of this investigation was to evaluate overall DNA damage induced by experimental paradoxical sleep deprivation (PSD) in estrous-cycling and ovariectomized female rats to examine possible hormonal involvement during DNA damage. Intact rats in different phases of the estrous cycle (proestrus, estrus, and diestrus) or ovariectomized female Wistar rats were subjected to PSD by the single platform technique for 96 h or were maintained for the equivalent period as controls in home-cages. After this period, peripheral blood and tissues (brain, liver, and heart) were collected to evaluate genetic damage using the single cell gel (comet) assay. the results showed that PSD caused extensive genotoxic effects in brain cells, as evident by increased DNA migration rates in rats exposed to PSD for 96 h when compared to negative control. This was observed for all phases of the estrous cycle indistinctly. in ovariectomized rats, PSD also led to DNA damage in brain cells. No significant statistically differences were detected in peripheral blood, the liver or heart for all groups analyzed. in conclusion, our data are consistent with the notion that genetic damage in the form of DNA breakage in brain cells induced by sleep deprivation overrides the effects related to endogenous female sex hormones. (c) 2009 Elsevier Inc. All rights reserved.
ISSN: 0018-506X
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Appears in Collections:Em verificação - Geral

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