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Title: Establishment and conventional cytogenetic characterization of three gastric cancer cell lines
Authors: Leal, Mariana Ferreira [UNIFESP]
Martins do Nascimento, Jose Luiz
Araujo da Silva, Carla Elvira
Vita Lamarao, Maria Fernanda
Calcagno, Danielle Queiroz
Khayat, Andre Salim [UNIFESP]
Assumpcao, Paulo Pimentel
Cabral, Isabel Rosa
Cardoso Smith, Marilia de Arruda [UNIFESP]
Burbano, Rommel Rodriguez
Universidade Federal de São Paulo (UNIFESP)
Fed Univ Para
Issue Date: 1-Nov-2009
Publisher: Elsevier B.V.
Citation: Cancer Genetics and Cytogenetics. New York: Elsevier B.V., v. 195, n. 1, p. 85-91, 2009.
Abstract: Gastric cancer is the fourth most frequent type of cancer and the second most frequent cause of cancer mortality worldwide. Only a modest number of gastric carcinoma cell lines have been isolated thus far. Here we describe the establishment and cytogenetic characterization of three new gastric cancer cell lines obtained from primary gastric adenocarcinoma (ACP02 and ACP03) and cancerous ascitic fluid (AGP01) of individuals from northern Brazil. ACP02, ACP03, and AGP01 cell lines are presently in the 60th passage. the cell lines grew in a disorganized single layer with some agglomerations and heterogeneous divisions (bipolar and multipolar). All cell lines exhibited a composite karyotype with several clonal chromosome alterations. Trisomy 8 was the most frequent alteration. Chromosome 8 aneusomy was confirmed by fluorescence in situ hybridization. All cell lines also exhibited trisomy 7 and deletion of chromosome arm 17p. These results suggest that, although frequent chromosome alterations are commonly observed due to culture process, the ACP02, ACP03, and AGP01 cell lines and primary gastric cancer from individuals of northern Brazil share genetic alterations, supporting use of these cell lines as a model of gastric carcinogenesis in this population. (C) 2009 Elsevier Inc. All rights reserved.
ISSN: 0165-4608
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