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|Title:||Invasiveness as a putative additional virulence mechanism of some atypical Enteropathogenic Escherichia coli strains with different uncommon intimin types|
|Authors:||Yamamoto, Denise [UNIFESP]|
Hernandes, Rodrigo T. [UNIFESP]
Schmidt, M. Alexander
Carneiro, Sylvia M.
Blanco, Jesus E.
Gomes, Tania Aparecida Tardelli [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Univ Santiago de Compostela
|Publisher:||Biomed Central Ltd|
|Citation:||Bmc Microbiology. London: Biomed Central Ltd, v. 9, 10 p., 2009.|
|Abstract:||Background: Enteropathogenic Escherichia coli (EPEC) produce attaching/effacing (A/E) lesions on eukaryotic cells mediated by the outer membrane adhesin intimin. EPEC are sub-grouped into typical (tEPEC) and atypical (aEPEC). We have recently demonstrated that aEPEC strain 1551-2 (serotype O non-typable, non-motile) invades HeLa cells by a process dependent on the expression of intimin sub-type omicron. in this study, we evaluated whether aEPEC strains expressing other intimin sub-types are also invasive using the quantitative gentamicin protection assay. We also evaluated whether aEPEC invade differentiated intestinal T84 cells.Results: Five of six strains invaded HeLa and T84 cells in a range of 13.3%-20.9% and 5.8%-17.8%, respectively, of the total cell-associated bacteria. the strains studied were significantly more invasive than prototype tEPEC strain E2348/69 (1.4% and 0.5% in HeLa and T84 cells, respectively). Invasiveness was confirmed by transmission electron microscopy. We also showed that invasion of HeLa cells by aEPEC 1551-2 depended on actin filaments, but not on microtubules. in addition, disruption of tight junctions enhanced its invasion efficiency in T84 cells, suggesting preferential invasion via a non-differentiated surface.Conclusion: Some aEPEC strains may invade intestinal cells in vitro with varying efficiencies and independently of the intimin sub-type.|
|Appears in Collections:||Em verificação - Geral|
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