Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/31563
Title: Critical involvement of Th1-related cytokines in renal injuries induced by ischemia and reperfusion
Authors: Paiva, Vanessa Nunes de [UNIFESP]
Monteiro, Rebecca M. M. [UNIFESP]
Marques, Vilmar de Paiva
Cenedeze, Marcos Antonio [UNIFESP]
Teixeira, Vicente de P. A.
Reis, Marlene A. dos
Pacheco-Silva, Alvaro [UNIFESP]
Camara, Niels O. S. [UNIFESP]
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Hosp Israelita Albert Einstein
Univ Fed Triangulo Mineiro
Keywords: Acute renal failure
Ischemia and reperfusion injury
Cytokines
IL-12
Interferon gamma
IL-6
HO-1
Issue Date: 1-Jun-2009
Publisher: Elsevier B.V.
Citation: International Immunopharmacology. Amsterdam: Elsevier B.V., v. 9, n. 6, p. 668-672, 2009.
Abstract: Renal ischemia and reperfusion injury (IRI) is considered an inflammatory syndrome. To move forward in its pathogenesis, we exploited the role of several cytokines on renal damages triggered by IRI. Specifically to evaluate the role of Th1 immune profile in this system, IL-12, IFN-gamma, and IFN-gamma/IL-12 deficient (KO) mice on C57BL/6 background and their controls were subjected to IRI. in each group, blood and kidney samples were harvested. Renal function was evaluated by serum creatinine and renal morphometric analyses. Gene expression of IL-6 and HO-1 were also investigated by Q-PCR. IFN-gamma KO animals presented the highest impairment in renal function compared to controls. Conversely, IL-12 KO animals were absolutely protected and, in a lesser extent, IFN-gamma/IL-12 KO double knockout was also protected from IRI. Gene expression analyses showed higher expression of HO-1, a cytoprotective gene, and IL-6, a pro-inflammatory cytokine, in IFN-gamma deficient animals subjected to IRI. Our results confirm that Th1 related cytokines such as IL-12 and IFN-gamma are critically involved in renal ischemia and reperfusion injury. (C) 2008 Elsevier B.V. All rights reserved.
URI: http://repositorio.unifesp.br/handle/11600/31563
ISSN: 1567-5769
Other Identifiers: http://dx.doi.org/10.1016/j.intimp.2008.11.012
Appears in Collections:Em verificação - Geral

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