Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/31326
Title: Short-Term Withdrawal of Mitogens Prior to Plating Increases Neuronal Differentiation of Human Neural Precursor Cells
Authors: Schwindt, Telma Tiemi [UNIFESP]
Motta, Fabiana Louise [UNIFESP]
Barnabe, Gabriela Filoso [UNIFESP]
Massant, Cristina Goncalves [UNIFESP]
Guimaraes, Alessander de Oliveira [UNIFESP]
Calcagnotto, Maria Elisa [UNIFESP]
Conceicao, Fabio Silva
Pesquero, Joao Bosco [UNIFESP]
Rehen, Stevens
Mello, Luiz E. [UNIFESP]
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio de Janeiro (UFRJ)
Issue Date: 27-Feb-2009
Publisher: Public Library Science
Citation: Plos One. San Francisco: Public Library Science, v. 4, n. 2, 10 p., 2009.
Abstract: Background: Human neural precursor cells (hNPC) are candidates for neural transplantation in a wide range of neurological disorders. Recently, much work has been done to determine how the environment for NPC culture in vitro may alter their plasticity. Epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2) are used to expand NPC; however, it is not clear if continuous exposure to mitogens may abrogate their subsequent differentiation. Here we evaluated if short-term removal of FGF-2 and EGF prior to plating may improve hNPC differentiation into neurons.Principal Findings: We demonstrate that culture of neurospheres in suspension for 2 weeks without EGF-FGF-2 significantly increases neuronal differentiation and neurite extension when compared to cells cultured using standard protocols. in this condition, neurons were preferentially located in the core of the neurospheres instead of the shell. Moreover, after plating, neurons presented radial rather than randomly oriented and longer processes than controls, comprised mostly by neurons with short processes. These changes were followed by alterations in the expression of genes related to cell survival.Conclusions: These results show that EGF and FGF-2 removal affects NPC fate and plasticity. Taking into account that a three dimensional structure is essential for NPC differentiation, here we evaluated, for the first time, the effects of growth factors removal in whole neurospheres rather than in plated cell culture.
URI: http://repositorio.unifesp.br/handle/11600/31326
ISSN: 1932-6203
Other Identifiers: http://dx.doi.org/10.1371/journal.pone.0004642
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