Please use this identifier to cite or link to this item: https://repositorio.unifesp.br/handle/11600/31292
Title: Endurance training induces depot-specific changes in IL-10/TNF-alpha ratio in rat adipose tissue
Authors: Lira, F. S.
Rosa, J. C. [UNIFESP]
Yamashita, A. S.
Koyama, C. H.
Batista, M. L.
Seelaender, M.
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Univ Mogi das Cruzes
Keywords: Endurance training
Adipose tissue
IL-10
TNF-alpha
PPAR-gamma
Issue Date: 1-Feb-2009
Publisher: Elsevier B.V.
Citation: Cytokine. London: Academic Press Ltd Elsevier B.V., v. 45, n. 2, p. 80-85, 2009.
Abstract: White adipose tissue (WAT) is the source of pro- and anti-inflammatory cytokines and recently, it has been recognized as an important source of interleukin 10 (IL-10). Acute physical exercise is known to induce an anti-inflammatory cytokine profile, however, the effect of chronic physical exercise on the production of IL-10 by WAT has never been examined. We assessed IL-10 and TNF-alpha concentration in WAT of rats engaged in endurance training. Animals were randomly assigned to either a sedentary control group (S, n = 7) or an endurance trained group (T, n = 8). Trained rats ran on a treadmill 5 days/wk for 8 wk (55-65% VO(2max). Detection of IL-10 and TNF-alpha protein and mRNA expression, as well as the gene expression of PPAR-gamma, and immunocytochemistry to detect mononuclear phagocytes were carried out. A reduction in absolute retroperitoneal adipose tissue (RPAT) weight in T (44%; p < 0.01), when compared with S was observed. IL-10 concentration was increased (1.5-fold, p < 0.05), to a higher extent than that of TNF-alpha (66%. p < 0.05) in the mesenteric adipose tissue (MEAT) of the trained group, while no change related to training was observed in RPAT. in MEAT, IL-10/TNF-alpha ratio was increased in T, when compared with S (30%; p < 0.05). PPAR-gamma gene expression was increased in T (1.1-fold; p < 0.01), when compared with S in the same adipose depot. No monocyte infiltration was found. in conclusion, exercise training induced increased IL-10 expression in the mesenteric depot, resulting in a modified IL-10/TNF-alpha ratio. We also conclude that WAT presents a depot-specific response to endurance training regarding the studied aspects. (C) 2008 Elsevier B.V. All rights reserved.
URI: http://repositorio.unifesp.br/handle/11600/31292
ISSN: 1043-4666
Other Identifiers: http://dx.doi.org/10.1016/j.cyto.2008.10.018
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