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Title: Characterization and functional analysis of the beta-1,3-glucanosyltransferase 3 of the human pathogenic fungus Paracoccidioides brasiliensis
Authors: Castro, Nadya da Silva
Castro, Kelly Pacheco de
Orlandi, Ivan
Feitosa, Luciano dos Santos [UNIFESP]
Rosa e Silva, Livia Kmetzsch
Vainstein, Marilene Henning
Bao, Sonia Nair
Vai, Marina
Almeida Soares, Celia Maria de
Universidade Federal de Goiás (UFG)
Univ Milano Bicocca
Universidade Federal de São Paulo (UNIFESP)
Univ Fed Rio Grande do Sul
Universidade de Brasília (UnB)
Keywords: Paracoccidioides brasiliensis
glycosylphosphatidylinositol anchored
cell wall biosynthesis
Issue Date: 1-Feb-2009
Publisher: Wiley-Blackwell
Citation: Fems Yeast Research. Hoboken: Wiley-Blackwell, v. 9, n. 1, p. 103-114, 2009.
Abstract: The fungus Paracoccidioides brasiliensis causes paracoccidioidomycosis, a systemic granulomatous mycosis prevalent in Latin America. in an effort to elucidate the molecular mechanisms involved in fungus cell wall assembly and morphogenesis, beta-1,3-glucanosyltransferase 3 (PbGel3p) is presented here. PbGel3p presented functional similarity to the glucan-elongating/glycophospholipid-anchored surface/pH-regulated /essential for pseudohyphal development protein families, which are involved in fungal cell wall biosynthesis and morphogenesis. the full-length cDNA and gene were obtained. Southern blot and in silico analysis suggested that there is one copy of the gene in P. brasiliensis. the recombinant PbGel3p was overexpressed in Escherichia coli, and a polyclonal antibody was obtained. the PbGEL3 mRNA, as well as the protein, was detected at the highest level in the mycelium phase. the protein was immunolocalized at the surface in both the mycelium and the yeast phases. We addressed the potential role of PbGel3p in cell wall biosynthesis and morphogenesis by assessing its ability to rescue the phenotype of the Saccharomyces cerevisiae gas1 Delta mutant. the results indicated that PbGel3p is a cell wall-associated protein that probably works as a beta-1,3-glucan elongase capable of mediating fungal cell wall integrity.
ISSN: 1567-1356
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